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《Acta Agriculturae Boreali-Sinica》 2010-S1
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Genetic Analysis and Evaluation of Agropyron. on ISSR

YAN Wei-hong,XU Zhu,LI Lin-hang,MA Yu-bao(Grassland Research Institute of Chinese Academy of Agriculture Science,Grassland Resource and Ecology Laboratory of Ministry of Agriculture,Huhhot 010010,China)  
The genetic structure of 12 Agropyron populations,including five species was analysed and evaluated by inter-simple sequence repeat(ISSR) markers.These materials were collected from six provinces in China,including Inner Mongolia,Hebei,Shanxi,Ningxia,Gansu and Jilin.ISSR-PCR amplification reaction system on Agropyron was been construct in China.The results showed that:A total of 88 fragments were amplified from 7 primers,of which 83 bands(94.32%) were polymorphic for the test materials,and there were a large amount of genetic polymorphism in the tested materials.Populations accessions of different species had different genetic richness.A.mongolicum had the most abundant genetic diversity.The second was A.cristatum.Genetic diversity of A.cristatum.var. pectinforme was relative lower than other species of Agropyron.The genetic variation found among species was 56. 35%and genetic diversity among regions is higher about A.cristatum.By UPGMA clustering,different populations of inter-species can be basically clustered together,but a few different populations of inter-species can't be clustered together.The species from similar eco-geographical situations have closer genetic distance and were clustered together.The genetic relationship among species can be speculated systematic relationship of five species on Agropyron.
【Key Words】： Agropyron. ISSR Genetic analysis Populations
【Fund】： “973”计划项目(2007CB106806);; 内蒙古半干旱草原区天然草地改良与人工饲料基地建设技术集成与示范(2006BAD16B05-4)
【CateGory Index】： S543.9
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