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《Acta Agriculturae Boreali-Sinica》 2010-S2
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Plant Expression Construct Based on RNAi for Soybean Mosaic Virus

XU Zong-hong1,2,3,HAO Qing-nan1,2,CHEN Li-miao1,2,SUN Dian-chen1,2,TIAN Xing-xing1,2,SHAN Zhi-hui1,2(1.Oil Crops Research Institute,Chinese Academy of Agriculture Sciences,Wuhan 430062,China;2.Key Laboratory of Oil Crop Biology,Ministry of Agriculture,Wuhan 430062,China;3.Yangtze University,Jingzhou 434023,China)  
Soybean Mosaic Virus(SMV) is belonging to Potyvirus.The disease caused by SMV is very popular,results in 10%-30% yield loss and exerts heave impact on soybean yield and quality.The pathogenicity of different virus strains varied with their nucleotide sequences.The utility of resistant varieties is the most economic pathway to control SMV.The resistant lines derived from conventional breeding material have narrow resistance spectrum,the resistance loss easily with the mutation of SMV strains.Acquired resistance based on RNA inference(RNAi) is a novel strategy to improve the plant resistance to virus.In this study,SMV isolate from Wuhan field was made sequence analysis,CP and Nib fragment were isolated based on homologous PCR,the fragment in most conserved region was chosed to construct RNAi vectors by use of gateway technique.The construct will be used in further transformation to improve soybean resistance to SMV.
【Fund】: 国家“863”计划(2007AA10Z416)
【CateGory Index】: S435.651
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