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《Acta Agriculturae Boreali-Sinica》 2011-02
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Construction of Biosafe Vectors of Resveratrol Synthase Gene and Rice Genetic Transformation

LI Hai-qing1,2,3,LIU Xu2,3,WANG Qing-guo2,3,YAO Fang-yin2,3,LIU Wei1,2,3(1.High-Tech Research Center,Shandong Academy of Agricultural Sciences,Key Laboratory of Crop GeneticImprovement and Biotechnology,Shandong Province,Jinan 250100,China;2.Key Laboratory of Crop GeneticImprovement and Biotechnology,Huanghuaihai,Ministry of Agriculture,Jinan 250100,China;3.College of Life Sciences,Shandong Normal University,Jinan 250014,China)  
Resveratrol is a kind of phytoalexins which can defence pathogen infection when suffered stresses.And resveratrol synthase,a kind of rate-limiting enzymes in Res synthesis pathway,plays key role during Res synthesis procedure.Rice blast,which caused by Magnaporthe grisea,is an extremely hazardous and widely distributed fungus disease.In this study,cDNA of resveratrol synthase gene was isolated using peanut total RNA as template by RT-PCR amplification.The plant expression binary vector of RS was then constructed using modified pCAMBIA1300 as framework plasmid which using maize ubiquitin promoter and bio-security screening marker bar instead of 35S promoter and Hygromycin by genetic engineering.The mature embyro of rice Shengdao 13 were transformed by the recombinated vector through agrobacterium-mediated transformation,and the positive transformants were further screened by Basta and PCR analysis.The work above will provided new materials for RS gene function identification,and shed some light for new rice germplasm innovation of disease resistence in the future.
【Fund】: 国家转基因植物新品种培育重大专项(2009ZX08001-010B)
【CateGory Index】: S511
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