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《ACTA AGRICULTURAE BOREALL—SINICA》 1994-03
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CMV_(cp) Gene Transformation and Expression in Pepper

Zhang Zongjiang;Zhou Zhongxin;Liu Yanjun(Tianjin Agricultural College,300381)Jiang Qianyun;You Ming(Tianjin Garden Bureau,Tianjin 300022)(Tianjing No 67 Middle School,Tianjin,300100)Liu Guomin;Mi Jingjiu(Tianjin Teachers,Training College)(Beijing Agricult  
Cotyledon sxplants from three pepper cultivars were co-cultured with an agrobacterium strain carrying a binary Ti vector in which a foreign CMVcp gene was inserted after the 35 spromotor After kanamycin-resistent selection and regeneration culture five transgeneplants and their offsprings were obtained. The transformation rate is 10%.Results of Southern and Northern Blots as well as ELISA tests indicated that the CMVcp gene has incorperated, transcripted and translated in transgeneplants and their offsprings.Virus inoculation tests prove the resistance of the transgeneplants and their offsprings to CMV virus.
【CateGory Index】: S188
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