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A Study on the Taxonomy and Relationship of Wild VitisNative to China with POD Isozyme

Ma Zhisheng(Shijiazhuang Pomology Institute,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050061)He Puchao(Northwestern Agricultural University,Yangling 712100)  
Peroxidase(POD)was used together with polyacrylamide gel electrophoresis (PAGE) in the tests of 76 clone of 12 wild vitis species and varieties native to China.The results are as follows:①A basic zymogram of POD isozyme was drown based on functional leaves of vitis,and 35 isoPOD bands were found in the zymogram. ②12 species and varieties were divided into 3 groups on the basis of the POD isozyme zymogram.③The analysis of the isozyme zymogram and the cluster analysis suggested that V.davidii,V.romanetii,V.piasezkii and V.psedoreticulata belonged to the primitive category,and V.amurensis,V.yeshanensis and V.adstricta evolutional category.④The grouping and evolution of species was related to their geographical distribution.
【Fund】: 国家自然科学基金
【CateGory Index】: S663.1
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