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Determination of β-carotene in Different Pumpkin Varieties by HPLC

Wu Zengru Jin Tongming (Beijing Vegetable Research Center,Beijing \ 100081)  
β-carotene from pulps of 31 varieties of pumpkin were determined by reversed phase high performance liquid chromatography (RP HPLC) with ultraviolet (UV) detector(254nm),a C_18 column and an eluant of 90% methanol and ethyl acetate.Dried powder samples were extracted by petroleum ether,evaporated to dryness and then dissolved by hexane to a final ratio of 1g of dried sample per 10ml hexane.The hexane solutions were analysed by HPLC.Difference of contents of β carotene between pumpkin varieties were found significant.Content of β carotene in spanish pumpkin was 8 735mg/100g .F.W.,which was far higher than that of other pumpkin varieties and even higher than the content of β carotene in carrot.Spanish pumpkin can be considered as a β carotene rich vegetable.
【CateGory Index】: S642.1
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