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《ACTA AGRICULTURAE BOREALL—SINICA》 1999-01
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Protoplast Culture and Plant Regeneration in Upland Cotton

Lü Fubing Zhang Xianlong Liu Jinlan (State Key Laboratory in Genetic Improvement of Crops,Huazhong Agricultural University,Wuhan 430070)  
In callus induction of Gossypium hirsutum L.cv.coker 201,IAA and 2,4 D showed positive effect and KT showed negative effect,while negative effect was shown with the three hormones in suspensions.The best enzymatic combination in protoplast isolation from embryogenetic suspensions was 3% cellulase(Onozuka R10)+1.5%pectinase,and the best hormone combination in protoplast culture was IAA 0.5mg/L+KT 0.1mg/L. Appropriately increasing protoplast density benefited protoplast culture. Light and temprature affected the protoplast culture.Protoplast derived calli growed rapidly in suspension culture medium without any hormone and regenerated plants through differentiation culture.
【CateGory Index】: S562.035.3
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