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《Hubei Agricultural Sciences》 2009-01
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Study on the Different Expression of Specific Proteins during Somatic Embrogenesis of Gossypium hirsutum L.

GUO Min-min1,2,WANG Qing-lian2,HU Gen-hai2(1.Henan Normal University,Xinxiang 453007,Henan,China;2.Henan Institute of Science and Technology,Xinxiang 453003,Henan,China)  
The dynamic changes of soluble proteins occurred during somatic embryogenesis of Gossypium hirsutum L.were an-alyzed,and the protein components occurred in the different development stages were compared by SDS-PAGE technique.The result indicated that the content of soluble protein changed regularly,being lower in earlier callus and reaching maxi-mum after two times of successive transfer culture,then decreasing.Specific proteins were detected at different development stages of embryogenesis.The proteins differed among the explants of different cultivars,which meant an important relations with its regeneration capacity.
【Fund】: 国家“十一五”科技支撑计划项目(2006BAD01A05-27);; 国家现代化产业技术体系项目(nycytx-06-09);; 河南省杰出人才计划项目(084200510008)
【CateGory Index】: S562
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