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《Hubei Agricultural Sciences》 2012-17
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Determination of Total Saponins in Robinia pseudoacacia L. Flowers by Colorimetry

JI Hong-fang1,ZHANG Ling-wen1,2,YANG Ming-duo2,LI Bin1,YAN Zhen-min1,ZHANG Fan1(1.School of Food Science,Henan Institute of Science and Technology,Xinxiang 453003,Henan,China;2.Postdoctoral Research Base of the Chinese Fast Food Research and Development Center,Harbin University of Commerce,Harbin 150076,China)  
Study was carried out to establish the method of determining saponins content in Robinia pseudoacacia L.flowers(RPF) by colorimetry.With Ginsenoside Rg1 as standard material,the condition of vanillin-perchloric acid colorimetry was optimized by orthogonal design.The suitable conditions for colorimetry were obtained as follows : the sample solution was evaporated by water bath heating at 60 ℃,and was mixed with 0.2 mL acetic acid containing 7.0% vanillin and 1 mL perchloric acid,and reaction mixture was incubated at 60 ℃ for 20 min.Detection wavelength was 545 nm.A good linear relationship was achieved in the range of 0~0.24 mg with the regression equation of y=3.547 0x+0.010 9(R2=0.998 9),and the average recovery was 100.07%(RSD=2.10%,n=7).The average content of total saponins in RPF was 2.177%.This method was simple,accurate,excellently stable and repeatable,and was suitable for the determination of total saponins in RPF.
【Fund】: 河南科技学院高层次人才科研项目(7041)
【CateGory Index】: R284
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