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《Hubei Agricultural Sciences》 2013-01
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Study on the Extraction of Icariine with Ethanol Refluxing Method and Ethanol Ultrasonic Method

SHI Juan(School of Chemical and Environmental Sciences,Shaanxi University of Technology,Hanzhong 723001,Shaanxi,China)  
Icariine was extracted from Epimedium through ethanol refluxing method and ethanol ultrasonic methods using ethanol as extract solvent.The effects of extraction times,extraction time,material to liquid ratio and ethanol volume fraction on extraction yield were studied by single factor experiments;and the extraction procedure was optimized by orthogonal design.The results showed that the optimized conditions of ethanol refluxing method were,material to liquid ratio,1∶10(m∶V,g/mL);refluxing time,40 min;extract times,twice;and ethanol volume fraction,75%.The optimized conditions for ultrasonic method were ethanol volume fraction,60%;ultrasonic time,30 min;extracting once,and material to liquid ratio,1∶6(m∶V,g/mL).
【Key Words】: icariine ethanol refluxing method ethanol ultrasonic method
【Fund】: 陕西省教育厅2012年科学研究项目(12JK0633);; 广元市科技局2011年科技支撑项目(2011GYKJ)
【CateGory Index】: TQ461
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【Citations】
Chinese Journal Full-text Database 6 Hits
1 ZHANG Jun-ying,HUANG Luo-sheng,CHEN Jian 1,CHEN Jie(TCM college of CPU, nanjing 210038, China);Study on Alcohol Extraction Process of Herba Epimedii[J];Strait Pharmaceutical Journal;2004-01
2 GAO Leng,BAO Lei,WANG Gu-yuan,GONG Dian-peng (School of Chemistry and Life Science,Changchun University of Technology,Changchun 130012,China);Ultrasound extraction process of Herba epimedii[J];Chemical Engineer;2010-02
3 ZHENG Chuan-chi, LOU Fang-ming, CHEN Gui-hua, HUA De-hong(Zunyi Medical College, Zunyi 563003, China);Optimization of Microwave Assisted Extraction Technology of Icariin from Epimedii Folium[J];China Pharmacy;2010-31
4 BAO Huilan1,CHEN Li2(1.Department of Endocrinology,Jinhua People's Hospital,Jinhua 321000,China;2.Dongyang Hospital of Traditional Chinese Medicine,Dongyang 322100,China);Icariin reduces mitochondrial oxidative stress injury in diabetic rat hearts[J];China Journal of Chinese Materia Medica;2011-11
5 ZHAO Lian-Mei,JI Xin,PAN Xiao-Ming,ZHANG Qian,HAN Li-Na,SHAN Bao-En.Research Center,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China;Immunoenhancement effect of ICA on the mouse model of Cy-induced hypoimmunity[J];Chinese Journal of Immunology;2009-12
6 WU Hao,ZHA Zhen-gang,YAO Ping,et al Department of Orthopedics,First Affiliated Hospital,Medical College of Jinan University,Guangzhou (510630);Experimental Study of Icariin in Inducing Bone Marrow Mesenchymal Stem Cell Differentiation[J];Chinese Journal of Integrated Traditional and Western Medicine;2010-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 GAO Meng-ting et al(College of Pharmacy and Chemistry,Dali University,Dali,Yunnan 671000);Research Advance in the Pharmacological Effects of Icariin[J];Journal of Anhui Agricultural Sciences;2012-18
2 ZHANG Lin1,KANG Bei-bei2,LI Ran1,FAN Ying1(1Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;2Department of Pediatrics,Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China);Discussion of research ideas on the effects of Chinese medicine on the osteoblast differentiation of bone marrow mesenchymal stem cells[J];China Journal of Traditional Chinese Medicine and Pharmacy;2012-08
3 MENG Ning,KONG Kai,LI Shi-weng(School of Chemical and Biological Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China);Advances in Studies on Chemical Constituents and Pharmaceutical Activities in Species of Epimedium[J];Acta Botanica Boreali-Occidentalia Sinica;2010-05
4 Xia Chen,Song Yan,Cao Yang,Zeng Xiaodan,Zhang Yingjiao(Institute of Agro-food Science and Technology,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China);Research on Ultrasound-assisted Extraction of Ginseng Polysaccharides from Ginseng Residue[J];Guangdong Chemical Industry;2012-01
5 JIANG Ling-yun1,LIU Guang-ming1,CHEN Ke-xin2 1.School of Pharmaceutical and Chemical,Dali University,Dali 671000,China 2.School of Basic Medicine,Dali University,Dali 671000,China;Advances in study on icariin extraction,separation,and its anti-tumor mechanism[J];Drugs & Clinic;2011-05
6 GU Yu-feng1,LIU Zhao-ying1,ZHANG Ming-jun1,ZHOU Si1,HUANG Ya-jun1, LI Yu-huan1,WU Yong1,SUN Zhi-liang2(1.Hunan Agricultural University Veterinary Faculty,Changsha 410128,China; 2.Hunan Engineering Research Center of Veterinary Drug,Changsha 410128,China);Determination of Dissolution Amount of Icariin in Different Particle Sizes of Epimedium by HPLC[J];China Animal Husbandry & Veterinary Medicine;2013-03
7 FENG Yan-jun,LIU Jing,LU Xiang(Shanghai Hospital of Traditional Chinese Medicine,Shanghai 200071,China);Epimedium Active Ingredient Antitumor-related New Progress[J];Journal of Liaoning University of Traditional Chinese Medicine;2012-07
8 ZHAO Lian'mei,PAN Xiaoming,SONG Huaqin,SHAN Bao'en Research Center,Fourth Hospital of Hebei Medical University,Shijiazhuang 05011,China;Icariin enhances the killing viability of CIK against uterine cervix cancer cells[J];Immunological Journal;2011-02
9 LIU Jing,YAN Ying,SHI Bing-nan,WANG De-hai(College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070);Study on effect of icariin on the immune function in mice[J];Heilongjiang Animal Science and Veterinary Medicine;2012-19
10 Mengting GAO, Fangfang MA, Shaohui WU, Tingshun LUO, Chenggui ZHANG, Guangming LIU College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China;Research Advance in the Pharmacological Effects of Icariin[J];药用植物研究(英文版);2013-01
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Di Kaijun Zhang Jingbo (Department of Cell Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China);Studies on the synergetic effect of Icariin and/or HMBA derivative on K562 cells[J];Oncology Progress;2003-01
2 MA Xiao-Qiong, WANG Long-Xing, XU Qing, ZHANG Feng, XIAO Hong-Bin, LIANG Xin-Miao * (Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China);Optimization Method of Multi-segment Gradient for Separation of Ginsenosides in Reversed Phase-High Performance Liquid Chromatography[J];Chemical Research In Chinese Universities;2004-02
3 LIANG Tong,ZhOU Ke yuan,LING Guang xin (Guangdong Medical College,Zhanjiang Guangdong 524023);Determination of Icariin in Epimedium and Its preparation by Micellar Electrokinetic Capillary Chromatography[J];Academic Journal of Guangdong College of Pharmacy;2000-02
4 GUO Tie-jun,BAI Hou-qiao, WEN Pei-e,REN Hai-quan,ZHANG Yu-kun,TANG Tian-hua, ZHANG Hao, BI Gao-feng, LIU Jia- ning, JIANG Feng- qin , JIANG Guo- sheng. Department of Hemato- Oncology , Shandong Academy of Medical Sciences, Jinan 250062, China;The change and significance of the signal transduction factor expression in HL-60 cells treated with ICA.[J];Journal of International Oncology;2006-04
5 SHI Yigang;TAO Tianzun;XU Qiang, et al The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China;Effect of TGF-β on the proliferation and differentiation and gene expression in rat osteoblast[J];Chinese Journal of Bone Tumor and Bone Disease;2003-03
6 Zhou Lifang(Department of Pharmacy PLA 95 Hospital,Putian);Determination of Icariin in Epimedium Grandiflorum by High Performance Capillary Electrophoresis[J];STRAIT PHARMACEUTICAL JOURNAL;1999-02
7 Guo Baolin; Xiao Peigen (Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences Beijing l00094);DETERMINATION OF FLAVONOIDS FROM EPIMEDIUML.IN SICHUAN[J];WEST CHINA JOURNAL OF PHARMACEUTCAL SCIENCES;1996-02
8 WANG Da-guang; ZHU Wen-yuan; MA Hui-jun(Department of Dermatology; the First Affiliated Hospital; Nanjing Medical University; Nanjing 210029; China );Effects of 6 kinds of monomers extracted from Chinese medicines including icariine onmelanogenesis in Cloudman S91 melanoma cells[J];Journal of Clinical Dermatology;2004-04
9 WANG Da-guang,ZHU Wen-yuan,MA Hui-jun,LI Cheng-rang,YUE Xue-zhuang(Department of Dermatology,the First Affiliated Hospital,Nanjing Medical University,Nanjing210029,China);Inhibitory effects of icariin on melanogenesis of human epidermal melanocytes[J];Journal of Clinical Dermatology;2004-08
10 Ge Lin fu,DONG Zheng jun,JIANG Guo sheng,et al.General Hospital of Jinan Command PLA,Jinan 250031,China;Experiment Study on the Effect of ICA on HL-60 Cells in Vivo and in Vitro[J];Journal of Qilu Oncology;2001-06
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