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《Hubei Agricultural Sciences》 2015-05
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Screening and Mutation of Chitinase-producing Strain

XIA Xiang;LV Meng-yuan;LUO Ling;CAI Jun;HU Ying;Key Laboratory of Fermentation Engineering(Ministry of Education)/Hubei Provincial Cooperative Innovation Center of Industrial Fermentation,Hubei University of Technology;  
A chitinase-producing strain was isolated and induced from various wild type microorganisms with colloidal chitin as the sole carbon source, and identified as Aeromonas sp. by 16 S r DNA. After first ultraviolet(UV) mutagensis, the highest chitinase production of the mutated strain was increased by 1.48 times. After the second UV mutagenesis or UV-Li Cl mutagenesis,the highest chitinase activity were increased by 3.16 and 3.86 times. The highest activity was increased to 3.48 U / m L. The strain HMX-16 obtained with the best activity had good genetic stability after UV and UV-Li Cl mutagenesis.
【Fund】: 湖北工业大学高层次人才启动项目(BSQD0813);; 2013年全国大学生创新创业训练计划项目(201210500030)
【CateGory Index】: TQ925;Q933
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