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《Journal of Hubei University for Nationalities(Natural Science Edition)》 2009-04
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Study on Tissue Culture Technique of Eastern Lilium Brownii for Rapid Propagation

ZOU Ying-chun1,QIN Da-ji1,WEI Dai-jun2,LONG Quan1,XIANG Ji-qian1,ZENG Fan-zhong1,YANG Yong-kang1,YIN Hong-qing1(1.West Comprehensive Experiment Station of Hubei Provincial Agriculture S&T Innovation Center,Enshi 445000,China;2.School of Science,Hubei University for Nationalities,Enshi 445000,China)  
Scales,leaves,stem segments and root tips of Eastern Lilium Brownii were used as ex-plant in the study.Mutual effects of different culture medium,growth regulators and/or different concentration were systematically studied and the most favorable media combination of culture factors for callus inducing and subculture for rapid propagation were selected in relevant experiment.Meanwhile,soilless culture technique research of plantlet and seed ball produced in vitro was also carried out.The experiment showed that: the best ex-plant was scales;the most favorable root inducing medium formula was MS+NAA(0.2 mg/L)+6-BA(2.0 mg/L),1/2MS+NAA0.3~0.5 for root growth proliferation.The best soilless culture medium for plantlet produced in vitro was the mixture of perlite,vermiculate peat with a proportion of 5∶1∶1.
【Fund】: 湖北省教育厅科研项目(B20092901)
【CateGory Index】: S682.29
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