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《Journal of Hubei Medical University》 2004-03
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A Simple and Rapid Method of MTT Assay for Evaluation of Antiviral Activity

Liu Zhao, Yang Zhanqiu, Xiao Hong, et al Institute of Virology, School of Medicine, Wuhan University, Wuhan 430071,China  
Objective: To established a rapid and sensitive method for evaluation of antiviral activity.Methods: The antiviral activity can be assesed as following: After the Hep 2 cells (at a dentisty of 2×10 5 seeded in 40 well tissue culture plates) were infected with CVB 3 and RSV and incubated for 1.5 h at 37 ℃. The virus were removed and different concentration of the test samples were added and incubatded again. When the cytopathic effect (CPE) could be observed at almost every test wells, 50 μl of the MTT solution (5 mg·ml -1 ) was added to each well. After 3 hours’ incubation, the MTT solution was removed and 100 μl DMSO was added to each well. 10 minutes later, the absorbance of the wells was recorded at 570 nm wavelengths for calculating the inhibitory rate of the virus.Results: The MTT assay was more simple, rapid, efficient, acute and objective than the CPE assay.Conclusion: The MTT assay can be widely applied to the screening and evaluation of anti virus substance.
【Fund】: 湖北省科技厅重点研究基金 ( 2 0 0 1ABB162 )
【CateGory Index】: R96
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