Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Hubei Medical University》 2004-03
Add to Favorite Get Latest Update

A Simple and Rapid Method of MTT Assay for Evaluation of Antiviral Activity

Liu Zhao, Yang Zhanqiu, Xiao Hong, et al Institute of Virology, School of Medicine, Wuhan University, Wuhan 430071,China  
Objective: To established a rapid and sensitive method for evaluation of antiviral activity.Methods: The antiviral activity can be assesed as following: After the Hep 2 cells (at a dentisty of 2×10 5 seeded in 40 well tissue culture plates) were infected with CVB 3 and RSV and incubated for 1.5 h at 37 ℃. The virus were removed and different concentration of the test samples were added and incubatded again. When the cytopathic effect (CPE) could be observed at almost every test wells, 50 μl of the MTT solution (5 mg·ml -1 ) was added to each well. After 3 hours’ incubation, the MTT solution was removed and 100 μl DMSO was added to each well. 10 minutes later, the absorbance of the wells was recorded at 570 nm wavelengths for calculating the inhibitory rate of the virus.Results: The MTT assay was more simple, rapid, efficient, acute and objective than the CPE assay.Conclusion: The MTT assay can be widely applied to the screening and evaluation of anti virus substance.
【Fund】: 湖北省科技厅重点研究基金 ( 2 0 0 1ABB162 )
【CateGory Index】: R96
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 GAO Yu-gang et al (Traditional Chinese Medicine College,Jilin Agricultural University,Changchun,Jilin 130118);Research on the Anti-virus of Antlers Polysaccharide[J];Journal of Anhui Agricultural Sciences;2010-22
2 CAI Bing-yan et al(Jiangsu Animal Husbandry & Veterinary College,Taizhou,Jiangsu 225300);Effects of Compound Acid Preparation against Newcastle Disease Virus in vitro[J];Journal of Anhui Agricultural Sciences;2010-26
3 LIN Hong1a,HU Ge1a,SUO Zhan-wei1a,YI Peng-fei2,HU Yi-yi3,MU Xiang1b(1.a.Department of Animal Science and Technology;b.Beijing Key Laboratory of VeterinaryMedicine Graditional Chinese Medicine and Pharmacology,Beijing University of Agriculture,Beijing,102206,China;2.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;3.Institute of Traditional Chinese Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China);Expression of IFN-γ of Pulmonary Microvascular Endothelial Cells Induced by Chinese Herbal Medicinal Constituents[J];Journal of Beijing University of Agriculture;2008-03
4 WANG Ai-ping1,ZHAN Song-he2,ZHU Liang-qiang1,QI Ke-zong1,YAO Xue-jing1(1.College of Animal Science and Technology,Anhui Agricultural University,Hefei,Anhui,230036,China;2.Anhui Animal Epidemic Disease Prevention and Control Center,Hefei,Anhui,230036,China);Inhibitive Effects of APS,RBV and IFN on PRRSV in vitro[J];Progress in Veterinary Medicine;2010-03
5 WEI Lan-ping,CHEN Jian-xin,HE Zi-hua,LU Chun-ni,LI Jin-feng,HUANG Xian-hui,FANG Bing-hu(College of Veterinary Medicine,South China Agricultural University,Guangzhou,Guangdong,510642,China);Effect of Hypericin on Influenza Virus H9N2 in Vitro[J];Progress in Veterinary Medicine;2010-12
6 FAN Rongjun LI Xiaopeng LIANG Shuang LIANG Ying HUAI Qingjie REN Lina Harbin Center for Disease Control and Prevention,Harbin 150056,China;The application of trace cell culture method in the EV71 virus research[J];China Medicine and Pharmacy;2012-11
7 CHANG Yang;ZHOU Youcai;DUAN Shumin;GONG Junxiang;Heilongjiang Aolida Ned Pharmaceutical Co., Ltd.;Heilongjiang Aolida Medical Group;Virus Diseases Prevention and Control Institute, China Center for Disease Prevention and Control;Heilongjiang Aolida Pharmaceutical Co., Ltd.;;Experimental study of inhibition and prevention effect of Niuhuang Qinggan capsules for H3N2 influenza virus A[J];China Medicine and Pharmacy;2013-16
8 XIE Xiu-chun;XU Jian-ya;WANG Shou-chuan;Nanjing University of Chinese Medicine;;Study on the effect of resveratrol against RSV in vitro[J];China Journal of Traditional Chinese Medicine and Pharmacy;2013-11
9 LU Gui-ping,CAI Bing-yan,LI Ju-yin(Jiangsu Animal Husbandry & Veterinary College,Taizhou 225300,Jiangsu,China);Anti-NDV Activity of Achyranthes bidentata Polysaccharide Sulfate In Vitro[J];Hubei Agricultural Sciences;2011-04
10 Liu Yuanyuan, Yang Zhanqiu, He Jing, et al Laboratory of Clinical Virology, Institute of Virology, School of Medicine, Wuhan University, Wuhan 430071, China;Comparison and Analysis of CPE Assay, MTT Assay and Plaque Reductive Assay for Evaluating Medicines in Antiviral Effects in vitro[J];Journal of Hubei Medical University;2005-02
China Proceedings of conference Full-text Database 2 Hits
1 GAO Yu-gang,LIU Jia-jia,YANG He,ZHANG Lian-xue,ZANG Pu (College of Chinese Traditional Medicine,Jilin Agricultural University,Changchun,Jilin,130118, China);The Screening of Chinese crude drug of Anti-bovine viral diarrhea virus from sika[A];[C];2009
2 Fan Jianhua~1,Xu Bu~1,Bu Shijin~2 (1.Poultry Institute,Chinese Academy of Agricultural Sciences,Yangzhou,Jiangsu 225125; 2.College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009);The Compound Preparation of Chinese Medicine,"Qingwen jiedu powder" to Pigeon Newcastle Disease Treatment Trials[A];[C];2013
【Citations】
Chinese Journal Full-text Database 4 Hits
1 Shen Yuan ying, Yang Zhan qiu, Liu Jian Jun,et al Institute of Virology, Medical College, Wuhan University, Wuhan 430071, China;Pathological Observation of Infection of Coxsakievirus B_3[J];Journal of Hubei Medical University;2001-04
2 TIAN Wen, SONG Shao bai, LU Hao ying, ZHENG Wei, TAN Hua, TANG Yun, WANG Yan, YU Yan xing (Department of General Surgery, PLA General Hospital, Beijing 100853);Studies of the effect of a fusion protein Fab/IFN αA on hepatitis B virus in vitro[J];ACADEMIC JOURNAL OF PLA POSTGRADUATE MEDICAL SCHOOL;2000-02
3 XU Mei ling,YU Jian er,PAN Xin,et al(28);Clinical and Experimental Study of “Pneumonia Formula” in Treating Coxsackie Virus Pneumonia[J];SHANGHAI JOURNAL OF TRADITIONAL CHINESE MEDICINE;2000-08
4 SUN Hongmei, LIU Zhewei, ZHANG Ting. Beijing Central Laboratory of Infection and Immunity,Capital Institute of Pediatrics, Beijing 100020,China;Antisense oligonucleotides resistance to coxsackievirus B_3 infection in HeLa cells[J];CHINESE JOURNAL OF EPIDEMIOLOGY;2000-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 SUN Ju-feng et al(Pharmaceutical College of Binzhou Medical College,Yantai,Shandong 264003);Research on Active Ingredients in Anti-virus Plants[J];Journal of Anhui Agricultural Sciences;2009-07
2 GUAN Feng-ying,LIU Yan,LI Hong,YANG Shi-jie(Department of Pharmacology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China);Protective effects of Astragalus Membranaceus injection on viral myocarditis in mice[J];Journal of Jilin University of (Medicine Edition);2005-05
3 Feng xin-shu Ao ya-zhou Xue cheng-yan,et al(Affiliated Hospital of Chengde Medical College,Hebei Chengde 067000,China);THE OBSERVATION OF EBV INFECTION AT PHARYNGEAL SECRETION AND TUMOR TISSUE OF LUNG CANCER PATIENTS ANS THEIR RELATION[J];Journal of Chengde Medical College;2005-04
4 YU Jie,XU You-hua(Children′s Hospital, Chongqing University of Medical Sciences,Chongqing 400014,China);Clinical study on the infection related neutropenia[J];Chongqing Medical Journal;2005-02
5 SUN Yun-yun1, JIANG Wen-lin2, LUO Xian-ling21Guangdong Cardiovascular Institute of Guangdong Province, Guangzhou 510100, China; 2Guangdong Geriatrics Institute of Guangdong Province, Guangzhou 510080, China;Detection of serum coxsackievirus B-specific antibody and nucleic acid in aged patients with cardiopathy[J];;2004-05
6 HOU Wei1,YANG Zhanqiu1,XIAO Hong1,QU Hong2,WEN Li1,MAO Lin1,LUO Jun3(1.Institute of Virology,School of Medicine of Wuhan University,Wuhan 430071,China;2.Department of Bioetiology,Tongji Medical College of Huazhong University of Science and Technology,Wuhan,Huber,430030,China;3.Guangzhou General hospital of Chinese PLA,Guangzhou,Guangdong,510010,China);Encephalitis of Herpes Simplex Virus on Mice[J];Progress In Veterinary Medicine;2003-02
7 JIANG Hong-xia, LIU Ya, SUN Ying-jun (Xuzhou Central Hospital of Jiangsu Province, Jiangsu Xuzhou 221009, China);Observation of the Effects of Reduning Combined with Pediatric Amino Acid on Infants with Rotavirus Enteritis[J];Journal of Pediatric Pharmacy;2009-06
8 WEI Fei,LIU Jing,YANG Zhan-qiu. State Key Laboratory of Virology,Institute of Medical Virology,Medical School Wuhan University,Wuhan 430071,China; LI Qing. Department of Basic Medicine,Xiamen University Medical College,Xiamen 361005,China;Anti-Rotavirus activity of four Rhubarb polysaccharides in vitro[J];Journal of Public Health and Preventive Medicine;2009-05
9 (PAN Yu-wei) PENG Yu-lan),(YAO Xu)(LI Cheng-rang) (Institute of Dermatology,Chinese Academy of Medical Sciences,Nanjing 210042,China);Outbreak of 4 cases of Kaposi varicelliform eruption in adults[J];Chinese Journal of Infection Control;2011-06
10 ZHANG Xiao-ying, ZHAO Zu-guo, WANG Xin-wei, GUO Jin-peng, CHEN Zhi-qiang, LI Jun-wen (Institute of Hygiene and Environmental Medicine, Academy of Military Medical Science, Tianjin 300050, China);Improvement of an Enteric Viruses Pick-up Method from Sewage[J];China Water & Wastewater;2006-10
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZUO Min1,NI Zhi-yu1,XU Li1,YU Feng1,LU Sa2,CHEN Shi-ping2,Hitota Mituru3,Suzuki Nobuo2,DONG Mei1(1.Faculty of Forensic Medicine,the School of Basic Medical Sciences,Hebei Medical Univercity,Shijiazhuang 050017,China;2.Department of Environmental Biochemistry,Graduate School of Medicine,Chiba University,Inohana 1-8-1,Chuoku,Chiba 260-8670,Japan;3.Department of Bioscience and Biotechnology,Faculty of Agriculture,Shinshu University,Nagano 399-4598,Japan);Anti-proliferative Activity and the Mechanisms of Euscaphis Japonica Kanitz in HeLa Cell Line[J];Carcinogenesis,Teratogenesis & Mutagenesis;2008-05
2 Xiao Kaijun,Chen Renju(College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,China);PREPARATION,APPARENT CHARACTERISTICS AND APPLICATION OF NEW PVAL ULTRFILTRATION MEMBRANE[J];Engineering Plastics Application;2009-11
3 DING Zhi-li et al(School of Life Science,Huzhou Teachers College,Huzhou,Zhejiang 313000);Isolation and Culture of Primordial Germ Cells of Chicken Embryo at 28th Stage[J];Journal of Anhui Agricultural Sciences;2009-02
4 WANG Xue-hong et al (Lanzhou Institute of Animal Science and Veterinary Pharmaceutics,The Chinese Academy of Agricultural Sciences,Lanzhou,Gansu 730050);Influence of Hypericum perforatum Extract on IFN-γ,TNF-α of Lung Tissue of Mice Infected with Influenza A Virus[J];Journal of Anhui Agricultural Sciences;2009-13
5 WEI Ti-man et al(College of Veterinary Medicine,Gansu Agriculture University,Lanzhou,Gansu 730070);Screening of Anti-Newcastle Disease Virus Ingredients of Phyllanthus urinaria[J];Journal of Anhui Agricultural Sciences;2009-18
6 DI Ting-ting et al (College of Animal Science and Technology,Inner Mongolia University for Nationalities,Tongliao,Inner Mongolia 028042);Research Progress on Duck Virus Hepatitis[J];Journal of Anhui Agricultural Sciences;2009-32
7 YangLiansongetal(RiceResearchInstitute,AnhuiAcademyofAgriculturalSciences,Hefei230031);AStudyonAntimicrobialActionandAntisepticEffectofTeaPolyphenolsonSomeCommonPutrefiedMicrobes[J];JOURNAL OF ANHUI AGRICULTURAL SCIENCES;1996-04
8 ZHANG Xiang Rong (Susong County Hospital, Anhui Province, Susong 246501);Progress in applications and studies of antiviral agents[J];Anhui Medical and Pharmaceutical Journal;2001-04
9 WangZhijie (Virus research institute,Hubei medical university,Wuhan 430071);THE PRIMARY INVESTIGATION OF EFFECTS ON HSV2 AND CVB3 VIRUS OF EMODIN OF RHIZOMA POLYGONI CUSPIDATI[J];JOURNAL OF ANHUI TRADITIONAL CHINESE MEDICAL COLLEGE;1999-03
10 LI Deng-hua TIAN Zhi-gang ZHANG JieZHANG Jian-hua CU Zheng-yan (Department of Immunology, Shandong Academy of Medical Sciences, Jinan 250001);THE USE OF MTT ASSAY FOR THE RAPID TESTING OF CYTOTOXICITY OF DRUGS AGAINST NON-SOLIDTUMOR CELLS[J];Chinese Journal of Cancer;1991-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 DAI Jian-hua et al(Jiangsu Animal Husbandry & Veterinary College,Taizhou,Jiangsu 225300);Inhibition IBDV Infecting CEF Activity Experiment of Rhodiola sachalinensis Polysaccharide in vitro[J];Journal of Anhui Agricultural Sciences;2011-34
2 CHENG QIAO De-mei et al(College of Chemistry and Materials Science,Northwest University,Xi'an,Shaanxi 710069);Overview of Pharmacological Research on Polygonum paleaceum Wall.[J];Journal of Anhui Agricultural Sciences;2012-15
3 LIN Hong1a,HU Ge1a,SUO Zhan-wei1a,YI Peng-fei2,HU Yi-yi3,MU Xiang1b(1.a.Department of Animal Science and Technology;b.Beijing Key Laboratory of VeterinaryMedicine Graditional Chinese Medicine and Pharmacology,Beijing University of Agriculture,Beijing,102206,China;2.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;3.Institute of Traditional Chinese Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China);Expression of IFN-γ of Pulmonary Microvascular Endothelial Cells Induced by Chinese Herbal Medicinal Constituents[J];Journal of Beijing University of Agriculture;2008-03
4 WANG Hui1 WU Sha1 SUN Yi-Fan1 JIANG Zhen-You1 LIAO Sen-Tai2 LIU Fan2(1Department of Microbiology and Immunology,School of Medicine,Jinan University,Guangzhou 510632,China;2Sericulture & Farm Produce Processing Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510610,China);An Assay on the Antiviral Activity of Mulberry Leaf Extract[J];Science of Sericulture;2011-05
5 Wei Feipeng,Tian Qiong,Li Xiaobing,Guo Weiping,Li Xubo,Liu Jingjing,Chen Juanjuan (1.Tangdu Hospital,The Fourth Military Medical University,Shanxi Xi'an 710038,China;2.The Fourth Military University,Shanxi Xi'an 710032,China);Empirical Study of Anti-RSV Effect on Gentiana Veitchiorum[J];Journal of Pediatric Pharmacy;2011-02
6 He Zhong-Mei1,Meng Xiang-Ying 1,Bao Yong-Li1,Wu Yin2,Zhu Hong-Yan1,Li Yu-Xin2 1(Institute of Genetic and Cytology,Northeast Normal University,Changchun 130024) 2(Research Center of Agriculture and Medicinal Gene Engineering of Ministry of Education,Changchun 130024);Antivirus Effect of Volatile Oil from Senecio Cannabifolius Less. and Its Constituents Analysis[J];Chinese Journal of Analytical Chemistry;2007-10
7 YE Jin-song1,GUAN Wen-da2,ZHAO Sui-shan2,YANG Zi-feng3,WANG Yu-tao2, QIN Sheng2,WU Shi-guan2,PAN Si-hua2,ZHAN Yang-qing2,XIE Hui-jun4(1.The Minimally Invasive Surgery Center,The First Affiliated Hospital of Guangzhou Medical College,Guangzhou 510230,China; 2.National Key Laboratory of Respiratory Diseases,First Affilated Hospital of Guangzhou Medical Collage,Guangzhou 510120,China; 3.Faculty of Chinese Medicine,Macao University of Science and Technology,Macao Special Administrative Region 00853,China; 4.Tropical Medicine Institute,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,China);Study on mechanism of antiviral effect of resveratrol against coxsackie virus in vitro[J];Guangdong Agricultural Sciences;2011-14
8 HUANG Pan,HAN Bao-qin,LIU Wan-shun,CHANG Qing,DONG Wen(College of Marine Life Science,Ocean University of China,Qingdao 266003,China);Preparation and Biocompatibility of N-carboxymethyl chitosan[J];Journal of Functional Materials;2009-07
9 YIN Pei-hui,HE Jian-hua,WANG Jian-hui,DONG Wei(Institute of Animal Nutrition,Hunan Agricultural University,Changsha 410128,China);Study On Anti-NDV Capacity of Six Different Tung-seed Meal Extracts in Vivo and in Vitro[J];China Animal Husbandry & Veterinary Medicine;2006-10
10 HE Yu-qin1,2,LIN Biao-sheng1,2,QIU long-xin1,3,DAI Ai-ling1,3,YANG Xiao-yan1,3(1.College of Life Sciences,Longyan University;2.Key Laboratory of Fujian University in Preventive Veterinary Medicine and Biotechnology;3.Fujian Provincial Engineering Research Center for the Prevention and Control of Zoonosis,Longyan,Fujian 364000,China);Antimicrobial activity of extracts from Anoectochilus roxburghii in vitro and its inhibitory effect on the proliferation of chicken NDV in CEF[J];Journal of Fujian Agriculture and Forestry University(Natural Science Edition);2012-04
China Proceedings of conference Full-text Database 1 Hits
1 ;GC-MS Analysis of the Low polarity components from Polygonum paleaceum Wall[A];[C];2013
【Secondary Citations】
Chinese Journal Full-text Database 7 Hits
1 Chen Zhongbin Wang Shengqi Zhu Baozhen Sun Zhixian (Department of Biochemistry and Molecular Biology,Institute of Radiation Medicine,Beijing 100850);STUDIES ON in Vitro ANTI-INFLUENZA A VIRUS ACTIVITY OF ANTISENSE PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDES[J];CHINESE JOURNAL OF VIROLOGY;1998-03
2 Zheng Wei, Tan Hua, Song Shaobai, Lu Haoying, Wang Yan, Yin Ru, Yu Yanxing (Department of General Surgery, General Hospital of PLA, Beijing 100853);The construction of expression vector for antiHBsAg Fab fragment and interferonαA fusion protein in E.coli[J];ACADEMIC JOURNAL OF PLA POSTGRADUATE MEDICAL SCHOOL;1998-03
3 Liu Jingxing, et al Department of Microbiology, Shanghai Second Medical University, Shanghai;Preliminary Studies on the Role of Anti Coxsackie Group B Virus of Chinese Medicine Sophora Flavescens[J];Acta Universitatis Medicinalis Secondae Shanghai;1991-02
4 Zhu Li Liu Jingxing Qian Furong, Department of Microbiology,Shanghai Second Medical University,Shanghai(200025);Measurement of Interferon Induced by Sophora Flavescens Alkaloids in Mouse Spleen Cells[J];Acta Universitatis Medicinalis Secondae Shanghai;1998-03
5 YT Zhang;NQ Wang;N Li;T Liu;ZW Dong Bejing Institute for Cancer Research, Beijing 100034;THE ANTITUMOR DFFECT OF ADRIAMYCIN CONJUGATED WITH MONOCLONAL ANTIBODY AGAINST GASTRIC CANCER IN VITRO AND IN VIVO[J];Acta Pharmaceutica Sinica;1992-05
6 ZhangQun,LiuWei,LianGuangLi,etalThechildren'shospitalofChongWenDistrict,Beijing100061;ClinicalandimmunoglobulinobservationofinfantswithCoxBviruspneumonia[J];CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY;1996-03
7 Wang manxia\ Lu changan\ Sun gang (Institute of Basic Theory,China Academy of TCM,Beijing 100700);Experimental Study on Anti HIV and Other Viruses by GO889,an Extract of Bulbus Allii[J];CHINA JOURNAL OF BASIC MEDICINE IN TRADITIONAL CHINESE MEDICINE;1999-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved