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Expression of Shh and Its Recepters mRNA in the Late Tooth Development of Murine Lower Incisor

Zhang Lu,Sun Zhijun,Wang Zhifeng,et alKey Laboratory for Oral Biomedical Engineering,School of Stomatology,Wuhan University,Wuhan 430079,China  
Objective: To investigate the temporal and spatial expression of Shh and its receptors Ptc1、Ptc2 mRNA in the late tooth development of murine lower incisor and to discuss its role in cyto diff erentiation.Methods: The embryonic mouse mandibles of late incisor development(E18.5~P 1.5 ) were obtained and 5μm serial sections were made. DIG labeling RNA probes of Shh,Ptc1 and Ptc2 were generated by in vitro transcription from Shh、Ptc1、Ptc2 cDNA template. The expression pattern of Shh,Ptc1 and Ptc2 mRNA was analyzed by means of in situ hybridization. Results: The expression pattern of Shh and Ptc2 was similar. In the late tooth development,they were expressed in the inner dental epithelium and stratum intermedium of the incisor labial side. Ptc1 mRNA was expressed in the inner dental epithelium and stratum intermedium as well as the odontoblast. Conclusion: In the late incisor development,Shh might participate in the ameloblast and odontoblast differentiation as a paracrine or autocrine signaling molecule.
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