Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Hubei Medical University》 2004-06
Add to Favorite Get Latest Update

Effect of Basic Fibroblast Growth Factor on Formation of Vascular Tube in Three-Dimensional Cell Culture

Zhang Lijun, Wang Zesheng, Zhang Lifeng Department of Geriatrics, Renmin Hospital, Wuhan University, Wuhan 430060, China  
Objective: To observe effect of basic fibroblast growth factor (bFGF) on the formulation of tubular structures in a three-dimensional cell culture system. Methods: Endothelial cells were cultured in a three-dimensional system on collagen gel surface through the stimulation of bFGF. Results: In bFGF group, endothelial cells grew in multi-layer soak type and transmuted into flat cells. Endothelial cells connected to collagen through hemidesmosome and themselves connected with each other through desmosome to form vascular tubes. Vascular tubes had various sizes and there were many endothelia cells in them. Endothelial cells and their nucleus changed in shape. In control group, endothelial cells grew in single layer and did not form vascular tubes. Conclusion: The technique of three-dimensional cell culture is simple and practicable for observation of angiogenesis in vivo. It is found that bFGF in three-dimensional cell culture is helpful for forming tubular structures.
【CateGory Index】: R329.4
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 ZHAO Jing, HUANG Jiang-Sheng, YANG Ai-Jun, WANG Chen-Yu, LIU Wei, LI Min Institute of Pathology, Lanzhou University, Lanzhou, Gansu, 730000, P. R. China;Three-dimensional Cell Culture and Histology of Vasculogenic Mimicry in Hepatocellular Carcinoma[J];Chinese Journal of Cancer;2007-02
【Co-references】
Chinese Journal Full-text Database 5 Hits
1 HE Xiaoyan, ZHANG Min, CHEN Zhichao, YOU Yong, TIAN Lei, ZOU Ping. Department of Hematology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R.China;Expression of PPAR-γ and its apoptotic significance in lung cancer[J];Chinese Journal of Lung Cancer;2006-01
2 Kang Yi,Li Xiuling,Yang Yuxiu,Wang Tiancai 1(Department of Gastroenterology,Henan Province People 's Hospital,Zhengzhou 450003,1Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China);;THE DETACTION AND SIGNIFICANCE OF PPARγ IN DIFFERENT HEPATIC DISEASES[J];Chinese Journal of Histochemistry and Cytochemistry;2006-05
3 LIU Yun-hai,ZHONG Ying-li,REN Kai-qun,ZHOU Jian-lin,ZHANG Jian,Han Mei(Department of Biochemistry and Molecular Biology,Life Science College of Hunan Normal University,Changsha 410081,China);Optimization of Cell Transfection Conditions[J];Journal of Natural Science of Hunan Normal University;2004-02
4 LI Gong-jie, YANG Li, SHI Xiao-lin, LI Xiao-bin, ZHEN Xiao-ling, WANG Yue-hua. Radiology Department of 307 Hospital of PLA, Beijing 100039, China;Study of the relationship between morphological characteristics of tumor microvasculature and blood supply type of primary hepatocellular carcinoma observed under DSA[J];Journal of Interventional Radiology;2005-02
5 LU Bin-bin,YIN Yong-mei,WANG Zhao-xia,WANG Teng,DE Wei1,SHU Yong-qian*(Department of Oncology,the First Afilliated Hospital of NJMU,Nanjing 210029,China);Effects of VEGF shRNA eukaryotic expression vector effect on human hepatocellular carcinoma cell line SMMC-7721[J];Acta Universitatis Medicinalis Nanjing(Natural Science);2006-06
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 SU Min1,HUANG Hua2,SUN Feng3,et al(1Department of Gynecology and Obstetrics,2 Department of Pathology,3Laboratory of Hematology,Affiliated Hospital of Nantong University,Nantong 226001);Morphologic observation of vasculogenic mimicry and expression of MMP2 in ovarian carcinoma[J];Acta Academiae Medicinae Nantong;2009-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved