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Effect of Basic Fibroblast Growth Factor on Formation of Vascular Tube in Three-Dimensional Cell Culture

Zhang Lijun, Wang Zesheng, Zhang Lifeng Department of Geriatrics, Renmin Hospital, Wuhan University, Wuhan 430060, China  
Objective: To observe effect of basic fibroblast growth factor (bFGF) on the formulation of tubular structures in a three-dimensional cell culture system. Methods: Endothelial cells were cultured in a three-dimensional system on collagen gel surface through the stimulation of bFGF. Results: In bFGF group, endothelial cells grew in multi-layer soak type and transmuted into flat cells. Endothelial cells connected to collagen through hemidesmosome and themselves connected with each other through desmosome to form vascular tubes. Vascular tubes had various sizes and there were many endothelia cells in them. Endothelial cells and their nucleus changed in shape. In control group, endothelial cells grew in single layer and did not form vascular tubes. Conclusion: The technique of three-dimensional cell culture is simple and practicable for observation of angiogenesis in vivo. It is found that bFGF in three-dimensional cell culture is helpful for forming tubular structures.
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