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Antiviral Activity of the Four Monomers of Isatis Tinctoria L Against Coxsackievirus B_3 in vitro

Zhao Lingmin, Yang Zhanqiu,Zhong Qiong, et al Institute of Virology, School of Medicine, Wuhan University, Wuhan 430071, China  
Objective: To investigate the antiviral activity of the four monomers of Isatis tinctoria L (the sixth, seventh, eighth, and ninth) on Coxsackievirus B 3 (CVB 3 ) in vitro. Methods: Anti-CVB 3 effects were investigated by observing cytopathic effect (CPE), by using MTT colorimetric assay for viable cell rate, and by detecting CVB 3 -RNA using semi-quantitative RT-PCR. Results: The four monomers of Isatis tinctoria L were more effective than the ribavirin in anti-biological synthesis of Isatis tinctoria L. As the dose of the drugs and the degree of CPE increased, the quantity of CVB 3 -RNA in culture media decreased and the viral inhibition rate increased. Conclusion: The four monomers of Isatis tinctoria L may inhibit CVB 3 biological synthesis but not inactivate the virus directly or block the adsorption to susceptible cells in vitro. Anti-CVB 3 effect of the four monomers was from best to worst as: the ninth monomer, the eighth monomer, the sixth monomer, and the seventh monomer.
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