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《Journal of Hubei Medical University》 2005-03
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Expression of Streptococcus Mutans Glucosyltransferase-B in Streptococcus Gordonii

He Li, Bian Zhuan,Zhang Qian School of Stomoatology, Wuhan University, Wuhan 430079, China  
Objective: To investigate the influences of Streptococcus mutans glucosyltransferase-B gtfB on the biochemical characteristics of streptococcus gordonii. Methods: E.coli-streptococcus shuttle plasmid pZB1 containing streptococcus mutans gtfB was transformed into streptococcus gordonii. Western blotting was used to determine whether gtfB was expressed in the transformant. The colony morphologies and in vitro sucrose-dependent adherence were detected to investigate the biochemical characters of streptococcus gordonii before and after being transformed.Results: The colony morphologies of streptococcus gordonii became smaller than the wild type. Western blotting results showed that one protein of the mutant reacting with the antibody against streptococcus mutans glucosyltransferase-B glucan-binding fragment had the same molecular weight as that of streptococcus mutans MT8148 cell-associated glucosyltransferase. In vitro sucrose-dependent adherence of streptococcus gordonii decreased significantly after being transformed. Conclusion: The expression of streptococcus mutans gtfB in streptococcus gordonii increased coaggregation of the cells.
【CateGory Index】: R378.1
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Chinese Journal Full-text Database 1 Hits
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