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《Medical Journal of Wuhan University》 2006-05
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Effects of GM1 on Heme Oxygenase-1 Expression in PC12 Cells During Oxygen Glucose Deprivation and the Role of Phosphatidylinositol 3-kinase/Akt Pathway in the Course

TANG Beibei,YANG Guocheng,XIA Laju,MENG Xiangping,LIU Li,GAO XinghuaDept.of Biochemistry and Molecular Biology,School of Medicine,Wuhan University, Wuhan 430071,China  
Objective: To investigate the effects of Monosialoganglioside(GM1)on the heme oxygenase-1(HO-1)expression in PC12 cells during oxygen glucose deprivation(OGD),and to study the role of phosphatidylinositol 3-kinase(PI-3K)/Akt signaling pathway in this course.Methods: An ischemical model was induced by incubation of PC12 cells with 1 mmol/L Na_(2)S_(2)O_(4) and glucose-free medium for four hours.Cells were treated with differect dose(25-100 μmol/L)of GM1 at the same time and(or)pretreated with LY294002,an inhibitor of PI-3K during OGD.Expression of HO-1mRNA and protein was determined by RT-PCR and Western blot analysis.Results: In contrast with the control group and OGD group,GM1 could up-regulate markedly the expression of HO-1mRNA and protein during OGD.Moreover,there was characteristic dose-depended relationship between GM1 and the expression of HO-1 mRNA and protein.Pretreatment of cells with LY294002 could partially attenuated the ability of GM1 to up-regulate HO-1mRNA and protein expression.Conclusion: GM1 could up-regulate HO-1 mRNA and protein expression in PC12 cells during OGD,and PI-3K/Akt signaling pathway plays an important role in this course.
【CateGory Index】: R96
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