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《Medical Journal of Wuhan University》 2007-06
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Expression and Significance of PAI-1, u-PA, VEGF and CD34 in Acute Cerebral Ischemia Rats Based on Atherosclerosis

ZHANG Kejian1,!LIU Yumin2,ZHANG Li3,SUN Yu2,HE Li11Dept. of Hematology, 2Dept. of Neurology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China3Dept. of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China  
Objective: To investigate the effect of the expression of PAI-1, u-PA, VEGF and CD34 on local cerebral ischemia injury and peripheral autologous endothelial progenitor cells of different groups in acute cerebral ischemia rats based on atherosclerosis. Methods: Male Wistar rats were randomized into two groups. Group A were fed with normal diet and group B were fed with high fat diet to construct atherosclerosis models. Then MCAO/R model was induced. The neurological scales were evaluated after focal cerebral ischemia in rats. HE stain and immunohistochemistry were used to detect the pathologic change and the infiltration cells with positive expression of PAI-1, u-PA, VEGF, CD34 in the regions of ischemia. The percentages of positive expression of CD34 were detected by FACS to determine the level of peripheral blood endothelial progenitor cells(EPCs). Results: ① After the focal cerebral ischemia injury, cells with positive expression of PAI-1, u-PA, VEGF were found at 24 h. The expression of PAI-1 and VEGF in group B was more obvious higher than group A, while that of u-PA was reverse. ② In group B,the percentage of positive expression of CD34 increased (P0.05). Meanwhile, there were fewer CD34 positive cells found in both ischemia region and peripheral blood in group B than in group A. Conclusion: The high expression of PAI-1, u-PA and VEGF advances atherosclerosis formation and aggravates ischemia injury. The low expression of CD34 and the decrease of EPCs in peripheral blood reduces the effect of neovascularization.
【Fund】: 湖北省科技攻关计划项目(编号:2001AA307B05)
【CateGory Index】: R743.3
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