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《河北医学院学报》 1992-01
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CELL CULTURE AND GENOMIC ANALYSIS OF REASSORTANTS OF ROTAVIRUSES

Li Yongfang Wang Yukun Gu Baoliang Department of Microbiology  
In propagation of human rotaviruses (HRV) and induction of typical cytopathic effects (CPE) of infected cells, trypsin treatment and roller culture technics were of crucial importance During cloning a virus mixture mixed by HRV FH4232 with Simian Rotavirus SA11, two reassortants of FH4232 and SAll were obtained. From analysis on viral RNA, it was found that the 5th and 11th segments of reassortant RNA electropherotype were stemed from parental RNA of strain FH4232, and the others from SA11. Rotavirus reassortants generated in vitro might account for the variability of HRV in vivo. In addition, these human-animal rotavirus reassortants would be offered to promise as potential vaccine candidates.
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