Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Hebei Medical University》 1992-01
Add to Favorite Get Latest Update

CELL CULTURE AND GENOMIC ANALYSIS OF REASSORTANTS OF ROTAVIRUSES

Li Yongfang Wang Yukun Gu Baoliang Department of Microbiology  
In propagation of human rotaviruses (HRV) and induction of typical cytopathic effects (CPE) of infected cells, trypsin treatment and roller culture technics were of crucial importance During cloning a virus mixture mixed by HRV FH4232 with Simian Rotavirus SA11, two reassortants of FH4232 and SAll were obtained. From analysis on viral RNA, it was found that the 5th and 11th segments of reassortant RNA electropherotype were stemed from parental RNA of strain FH4232, and the others from SA11. Rotavirus reassortants generated in vitro might account for the variability of HRV in vivo. In addition, these human-animal rotavirus reassortants would be offered to promise as potential vaccine candidates.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 CHEN Jun-hua1,XIE Yuan-yuan1,LING Hua2,LIU Zuo-yi1(Department of Infection and Gastroenterology,Children's Hospital,Chongqing Medical University,Chongqing 400014;Chongqing Center for Disease Control and Prevention,Chongqing 400042,China);On pathogenesis of rotavirus diarrhea in suckling mouse[J];Acta Academiae Medicinae Militaris Tertiae;2007-12
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YAO Ying-min1, OU Qiao-qun1, CHEN Yao2 Department of Pediatrics, Nanfang Hospital1, School of Biotechnology2, Southern Medical University, Guangzhou 510515, China;Ultrastructural changes of the extraintestinal organs of newborn mice with human rotavirus infection[J];Journal of Southern Medical University;2006-09
2 Meng Jihong Zhang Jianqiong Lin Ling (Department of Microbiology,Nanjing Railway Medical College,Nanjing);STUDIES ON CULTIVATION OF ROTAVIRUS IN CELL CULTURE[J];Journal of Nanjing Railway Medical College;1993-02
3 WANG Peng yan, CHEN Chuang fu (College of Animal Science & Technology, Shihezi University, Shihezi 832003, China);Cell Culture of Porcine Rotavirus[J];Journal of Shihezi University(Natural Science);2002-04
4 SONG Yan1,WEI Li-li1,CHENG Zhi2(1. Lab of Molec. Biol. of Pathogen.; Coll. of Anim. Med. NE Agric. Univ. Harbin 150040;2. Teach. & Res. Sect. of Microbiol. Coll. of Basic Med.; Harbin Med. Univ. Harbin 150036);Isolation and Characterization of Human Rotavirus HRB-02 Strain[J];Journal of Microbiology;2005-01
5 YANG Mei-fen~1,HOU Zhong-liu~2,HUANG Yong-kun~1,LI Hai-ling~1(1.Department of Pediatrics,the First Affiliated Hospital of Kunming Medical College,Kunming 650032,China;2.Institutite of Medical Biology,Chinese Academy of Medical Sciences Biology,Kunming 650118,China);Experimental study on extraintestinal symptom of adult mouse with rotavirus infection[J];Chinese Journal of Microecology;2006-05
6 CHEN Shu-hong , WANG Xin-sheng , SHI Dong-fang , LI Yi-jing (College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China);Isolation and characterization of porcine rotavirus[J];Chinese Journal of Preventive Veterinary Medicine;2004-01
7 Zhou Zhengren, et al Dept of Microbiology;ISOLATION CULTURE OF ROTAVIRUS AND COMPARING STUDY OF THE DETECTING METHODS[J];Journal of China Medical University;1990-05
8 Yao Yingmin, Zhao Feng, Wu Jianchun, et al. Nanfang Hospital, Guangzhou 510515;A case report of systemic infection caused by rotavirus[J];Chinexe Journal of Pediatrics;1997-07
9 JI Shaozhong, BI Ye, YANG Hongyan, YANG Fengrong, SONG Junqi, TAO Xiaoxia, CUI Xiaoying Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206, China;Cultivation and serial propagation of a new rotavirus causing adult diarrhea in primary human embryo kidnay cells[J];National Medical Journal of China;2002-01
10 YAO Ying-min, OU Qiao-qun, CHEN Yao (Department of Pediatrics,Nanfang Hospital,Southern Medical University, Guangzhou,510515 , China );Pathological changes of extra-intestinal infection in newborn mice infected with human rotavirus[J];Chinese Journal of Zoonoses;2006-07
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 CHEN Jun-hua,XIE Yuan-yuan,ZHU Chao-min,LIU Zuo-yi(Department of Infectious Disease,Children's Hospital,Chongqing Medical University,Chongqing 400014,China);Extraintestinal lesions caused by rotavirus:An experimental study on suckling mice[J];Acta Academiae Medicinae Militaris Tertiae;2009-15
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved