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《JOURNAL OF HEBEI MEDICAL UNIVERSITY》 1998-01
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APPLICATION OF DIG LABELED cDNA PROBE TO DETECT INDUCED NITRIC OXIDE SYNTHETASE GENE EXPRESSION OF LUNG

Wang Junxia Wan Mei Cong Bin Ling YilingDepartment of Molecular Biology  
Objective:To detect the expression of induced nitric oxide synthetase(iNOS) gene in rabbit lung after endotoxin administration.Methods:By using Northern blot and colormetric technique,iNOS gene expression in rabbit lung was identified and iNOS cDNA probe was labeled by randomly primed incorporation of digoxigenin-dUTP.Results:After injection of LPS,the expression of iNOS gene showed most prominently 5 hours,then decreased in 24 hours.Conclusion:The DIG labeled probes may be useful in the study of iNOS gene expression.
【CateGory Index】: R332.2,R446.6
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