Effect of Shenfu injection with cytokines on the maturation and function of dendritic cells derived from AML HL-60 cells
REN Lili,WEN Ming,LI Shuqing,et al.*Affiliated Hospital of Hebei University,Hebei,Baoding 071000,China
Objective To investigate the effect of shenfu injection (SFI) with cytokines on the maturation and function of dendritic cells (DCs) derived from the HL-60 cells.Methods Different concentrations of SFI plus different concentrations of SFI or GM-CSF+IL-4,or GM-CSF+IL-4 were used to induce HL-60 cells getting DCs.The morphologic changes and the phenotypic expression rate were observed,at the same time,the capability of DCs stimulating T lymphocyte proliferation was tested.The level of INF-γ in supernatant was measured by ELISA method.Results Only SFI could not induce the HL-60 to derive DCs.While DCs could derive from HL-60 cells co-cultured with GM-CSF/IL-4 but it was immature.However,different concentration of SFI plus GM-CSF/IL-4 not only induced maturation of HL-60 DCs but also restrained propagation of cells.Compared with other control groups,cells cultured with middle concentration expressed higher levels of phenotypes,elicited significantly stronger abilities of stimulating T lymphocyte proliferation,and secreted larger amounts of INF-γ in supernatant.Conclusion Appropriate concentrations of SFI plus GM-CSF/IL-4 could effectively induce differentiation and maturation of HL-60 DCs,and enhance their immune stimulatory function.
【CateGory Index】： R733.71