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Classification of four Holotrichia spp. (Coleoptera: Melolonthidae) by RAPD

WANG Si fang, GU Yun, GU Song dong (Laiyang Agricultural College, Laiyang, Shandong 265200, China)  
RAPD technique was used to classify four Holotrichia spp.. The results showed that the average genetic distances between Wendeng population and Nanjing population of H.oblita was 0.346, Wendeng population and H.diomphalia 0.375, Nanjing population and H.diomphalia 0.359. The average genetic distances between H.ernesti and Wendeng population, Nanjing population, H.diomphalia were 0.553, 0.545, 0.534 respectively. Cluster analysis results showed that the four Holotrichia spp. were grouped into two clusters. Of the two clusters, one was H. ernesti , the other was H.oblita and H.diomphalia . All results showed that H.oblita and H.diomphalia were the identical species. According to Code Zoological Nomenclature, H.diomphalia is the synonym of H.oblita .
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