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Study on DNA Damage Induced by HgCl_2 and CdCl_2 Using SCGE in Eriocheir Sinensis Sperms(In Vitro)

FAN Li-jun,ZHOU Zhong-liang,CHEN Dong-hua,ZHAO Yun-long,SUN Ju-xiang,WANG Qun(School of Life Science,East China Normal University,Shanghai 200062,China)  
DNA damage induced by mercuric chloride(HgCl2) and cadmium chloride(CdCl2) in Eriocheir sinensis sperms(in vitro) was studied using single-cell gel electrophoresis(SCGE).Separated DNAs were dealt with six HgCl2 and CdCl2 scalar solutions(for each)(0 mmol/L,0.01 mmol/L,0.05 mmol/L,0.1 mmol/L,1 mmol/L,5 mmol/L) for one hour at 37 ℃(water bath) respectively.The length of cometic tail and damage rate of DNAs were betaken as SCGE indices.Results showed that DNAs in control group are free of damage,static in electric field and manifested as a round fluorophoreu without any cometic tails.While those dealt DNAs are damaged to various degrees and demonstrated as cometic fluorophoreu in electric field after Ethidium bromide(EB) stain.Furthermore,with the incremental concentration of HgCl2 and CdCl2,damage rate and migration distance also enlarge accordingly,that′s to say,doseresponse relationship exist between SCGE indices and concentration of HgCl2 and CdCl2.Therefore,it is concluded that certain concentration of HgCl2 and CdCl2 does induce DNA damage in Eriocheir sinensis sperms(in vitro) and the damage degree is related to the concentration of HgCl2 and CdCl2,which have been attested using SCGE.
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