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《Journal of East China Normal University(Natural Science)》 2007-04
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High Throughput Screening Method of Identifying Antagonist for Glucagaon Receptor in Chinese Herbs

WAN Juan, XU Zhi-liang(Key Laboratory of Brain Functional Genomics of Shanghai,School of Life Science,East China NormalUniversity,Shanghai 200062,China)  
This paper generated a cell-based functional assay for human glucagon receptor antagonist screening.The human glucagon receptor cDNA was cloned into a mammalian expression vector pCDNA3.1(h Glucagon receptor /pCDNA3.1).This plasmid was co-transfected with a reporter gene construct(3×CRE/3×MRE/SRE-LUC) in HEK293 cells.Stable cell line expressing both human glucagon receptor and the reporter gene was selected for compounds screening.The assay condition was optimized.Our results demonstrated that this cell-based assay can be optimized for high throughput screening and applied to identify antagonist for glucagon receptor in Chinese Herbs.
【Fund】: 国家科技部863计划项目(2003AA221061)
【CateGory Index】: R285
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