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《Journal of Hubei University(Natural Science)》 2007-01
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Cloning and hpRNA vector construction for fad2 genes in Brassica napus and Lesquerella fendleri

WANG Cheng-gang,WANG Wen-yan,XIE Yu,HUANG Bang-quan (1 School of Life Science,Hubei University,Wuhan 430062,China;2 Wuhan Bioengineering Institute,Wuhan 430415,China)  
Delta-12 desaurase(FAD2) plays an important role in the poly-unsaturated fatty acid synthesis.Gene fragments for fad2 was PCR-amplified from B.napus and L.fendleri genomic DNA and used as templates to amplify a smaller fragment.The larger and the smaller fragments of the same gene were ligated in opposite orientations into a seed specific expression vector 2300-nap to produce hpRNA vectors.Sequence analyses indicated that the fragments we cloned were indeed genes for fad2 in B.napus and L.fendleri.
【Fund】: 湖北省科技厅国际合作重点项目(2005CA020);; 湖北省教育厅国际合作项目(G200510001)资助
【CateGory Index】: Q943.2
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1 Wang Chenggang Zhang Juhong Xie Yu Liu Youqi Huang Bangquan*Lab of Plant Genetics and Breeding, College of Life Science, Hubei University, Wuhan, 430062;Cloning and hpRNAi Vector Construction of Brassica napus Pyrophosphate-dependent Phosphofructokinase (PFK)[J];Molecular Plant Breeding;2006-02
2 Li Jia; Shen Bingzhang; Han Jixiang; Gan Li (Huazhong Agricultural University, Wuhan 430070);A EFFECTIVE PROCEDURE FOR EXTRACTING TOTAL DNA IN RAPE[J];JOURNAL HUAZHONG(CENTRAL CHINA) AGRICULTURAL UNIVERSITY;1994-05
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【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Li Jia; Shen Bingzhang; Han Jixiang; Gan Li (Huazhong Agricultural University, Wuhan 430070);A EFFECTIVE PROCEDURE FOR EXTRACTING TOTAL DNA IN RAPE[J];JOURNAL HUAZHONG(CENTRAL CHINA) AGRICULTURAL UNIVERSITY;1994-05
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