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《Journal of Hubei University(Natural Science)》 2007-01
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Cloning and hpRNA vector construction for fad2 genes in Brassica napus and Lesquerella fendleri

WANG Cheng-gang,WANG Wen-yan,XIE Yu,HUANG Bang-quan (1 School of Life Science,Hubei University,Wuhan 430062,China;2 Wuhan Bioengineering Institute,Wuhan 430415,China)  
Delta-12 desaurase(FAD2) plays an important role in the poly-unsaturated fatty acid synthesis.Gene fragments for fad2 was PCR-amplified from B.napus and L.fendleri genomic DNA and used as templates to amplify a smaller fragment.The larger and the smaller fragments of the same gene were ligated in opposite orientations into a seed specific expression vector 2300-nap to produce hpRNA vectors.Sequence analyses indicated that the fragments we cloned were indeed genes for fad2 in B.napus and L.fendleri.
【Fund】: 湖北省科技厅国际合作重点项目(2005CA020);; 湖北省教育厅国际合作项目(G200510001)资助
【CateGory Index】: Q943.2
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 Wang Chenggang Zhang Juhong Xie Yu Liu Youqi Huang Bangquan*Lab of Plant Genetics and Breeding, College of Life Science, Hubei University, Wuhan, 430062;Cloning and hpRNAi Vector Construction of Brassica napus Pyrophosphate-dependent Phosphofructokinase (PFK)[J];Molecular Plant Breeding;2006-02
2 Li Jia; Shen Bingzhang; Han Jixiang; Gan Li (Huazhong Agricultural University, Wuhan 430070);A EFFECTIVE PROCEDURE FOR EXTRACTING TOTAL DNA IN RAPE[J];JOURNAL HUAZHONG(CENTRAL CHINA) AGRICULTURAL UNIVERSITY;1994-05
3 Shi Dongqiao, Zhou Yihua, Hu Zanmin, Zhang Lihua, Liu Guizhen, Chen Zhenghua (Institute of Genetics, Chinese Academy of Sciences, Beijing 100101);Introduction of trans Desaturase Gene into Brassica napus L. via ParticleBombardment and Obtaining of Transgenic Plants[J];Journal of Agricultural Biotechnology;2001-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Yawei et al (Department of Plant Protection,Hubei Agricultural College,Jingzhou,Hubei 434025);Study on Bioactivity of Stellera Chamaejasme Against Pieris Rapae[J];Journal of Anhui Agricultural Sciences;2004-02
2 XUE Xiao-ping et al(Guizhou Institute of Tobacco Science,Guiyang,Guizhou 550003);Observation on the Control Efficacy of Stellera chamaejasme Extract on Cucumber Mosaic Disease of Tobacco[J];Journal of Anhui Agricultural Sciences;2008-09
3 JI Ya-jun(Qinghai Academy of Animal Science and Veterinary Medicine,Xining,Qinghai 810016);Research Progress on Achnatherum inebrians[J];Journal of Anhui Agricultural Sciences;2009-05
4 MA Xiu-jie,ZHANG Yue-an,YANG Rui (Biology Department,Baicheng Normal College;Baicheng Education Institute,Baicheng 137000,China);A Primary Study on the Soil Microflora in Western Jilin[J];Journal of Baicheng Normal College;2009-03
5 WEN Zhe-yi,SHI Guang-lu,SU Xue-you,WU Jing,WANG You-nian* (Beijing Key Laboratory of New Technolgy of Agricultural Application,Beijing University of Agriculture,Beijing 102206,China);Biological Activity of Extract from the Root of Stellera chamaejasme on Fungi and Enzyme of Peach Fruits[J];Journal of Beijing University of Agriculture;2008-01
6 CHEN Mei1,SHI Guang-lu2,LI Zhao-hui1(1.College of Plant Protection,Shandong Agricaltural University Taian,Shandong 271028,China;2.Department of Biotechnology,Beijing University of Agricalture,Beijing 102206,China);Extractive Technic from Stellera chamaejasme by Supercritical Fluid Extraction and Its Biological Activities against Tetranychus cinnabarinus(Boisduval)[J];Journal of Beijing University of Agriculture;2008-03
7 SHI Da zhao 1,YANG Ai lian 2 (1.China Agricultural University,Beijing 100094,China; 2.National Station of Animal Husbandry and Veterinarian, Beijing 100026,China);Perfect Harmful Biological Early Warning System Promoting Grass for Sustained Development[J];Acta Agrestia Sinica;2002-04
8 ZHANG Xing-xu,CHEN Na,LI Chun-jie,NAN Zhi-biao (Key Laboratory of Grassland Argo-Ecosystem,Ministry of Agriculture;College of Pastoral Agriculture Science and Technology,Lanzhou University;Gansu Grassland Ecological Research Institute;Lanzhou,Gansu Province 730020,China);Physiological Effects of Rhopalosiphum padi on Achnatherum inebrians Keng Infected by Endophyte[J];Acta Agrestia Sinica;2008-03
9 WANG Shen-yao,WANG Kun,LA Dan(The grassland institution,Agricultural University China,Beijing);Density,longevity and viability of Oxytropis in soil seed bank in surrounding area of Qinghai Lake[J];Grassland and Turf;2008-06
10 YAO Tuo1,DU Wehua1,KANG Tianfang2(1 College of Grassland Science,Gansu Agricultural University,Lanzhou,730070,Gansu,China;2Department of Plant Protection, Gansu Agricultural University, Lanzhou, 730070, Gansu,China);Primary study on the harmfulness of Oxytropis and its control strategy[J];Pratacultural Science;2003-06
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Li Jia; Shen Bingzhang; Han Jixiang; Gan Li (Huazhong Agricultural University, Wuhan 430070);A EFFECTIVE PROCEDURE FOR EXTRACTING TOTAL DNA IN RAPE[J];JOURNAL HUAZHONG(CENTRAL CHINA) AGRICULTURAL UNIVERSITY;1994-05
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