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《Journal of Henan University(Natural Science)》 2010-05
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Study on Recognition Mechanism of Molecularly Imprinted Microsphere Using Rifampicin as Template

ZHANG Cheng-li1,ZHOU Yan-mei1,MA Tong-sen1,LEI Jian-du2 (1.Institute of Environmental and Analytical Sciences,College of Chemistry and Chemical Engineering,Henan University,Henan Kaifeng 475004,China;2.National Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100080)  
Molecular imprinted polymer microspheres were synthesized by aqueous suspension polymerization using polyvinyl alcohol 1735(PVA) as dispersive reagent,rifampicin(RFP) as template molecule,MAA as functional monomers and EGDMA as the crosslinker with high selectivity for RFP.The polymers were investigated in equilibrium binding experiments and Scatchard analysis to evaluate the binding characteristics and recognition mechanism of the RFP-molecular imprinted polymer in aqueous solution.Scatchard analysis showed that two binding sites were formed in the imprinted polymer matrix under the studied concentrations.The dissociation constant(Kd1) and the apparent maximum binding capacity(Qmax1) were Kd1=1.08×10-2 mg/mL,Qmax1=14.40 mg/g for high affinity binding sites and Kd2=7.18×10-3 mg/mL,Qmax2=12.51 mg/g for low affinity binding sites.The experiments of binding different substrates showed that the imprinted polymer possessed a high selectivity and a high recognition ability for RFP.The method has advantages of simpler operation and higher accuracy,and may be widely used.
【Fund】: 国家自然科学青年基金资助项目(20506026);; 国家高技术研究发展计划863资助项目(2006AA10Z437);; 河南省教育厅自然科学研究项目(2008B610001、2009A610003)
【CateGory Index】: O631.3
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