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《Natural Science Journal of Harbin Normal University》 2008-06
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CLONING AND SEQUENCE ANALYSIS OF OPEN READING FRAME OF psbD GENE IN LEYMUS CHINENSIS

Xu Jing(Harbin Normal University) Wang Aoxue (Northeast Agricultural University)Li XinlingXu Xiangling(Harbin Normal University)  
Total RNA was extracted from leaves of L.chinensis psbD gene open reading frames'(Open reading frame,ORF) cDNA sequences was amplified using degenerate PCR method.This gene encodes 353 amino acids,its GenBank Access No.is FJ176573.Meanwhile,its sequence was analyzed.The genomic DNA of psbD gene open reading frame was amplified.The psbD gene has no intron.This study will provide the gene material and theoretical basis for the further study of psbD gene's function.
【Fund】: 黑龙江省重大攻关课题(6A06B103-10)
【CateGory Index】: S543.9
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