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《哈尔滨工业大学学报(英文版)》 2007-05
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Length polymorphisms for intergenic spacer regions of 16S-23S rDNA in members of the new hydrogen-producing bacteria

LI Yong-feng1,2,3, XU Jing-li1, YANG Chuan-ping2, REN Nan-qi3 (1.Chemical Engineering School, Shanghai University of Engineering and Sciences, Shanghai 201620, China, 2.Forestry School, Northeast Forestry University, Harbin 150040, China; 3.Municipal and Environmental Engineering School,Harbin Institute of Technology, Harbin 150090,China)  
A method based on PCR amplification of the 16S rRNA gene (rDNA)-23S rDNA intergenic spacer regions (ISR) was developed for the identification of species within the novel group hydrogen-producing anaerobes. The sizes of the PCR products varied from 1264 to 398 bp. Strain of isolate Rennanqilyf 3 was characterized as having products of 1262,398,638,437 and 436 bp. The isolate Rennanqilyf 1 had product of 1264 bp. The isolate Rennanqilyf 13 had products of 1261,579 and 485 bp. Of the 3 species of the novel group hydrogen-producing anaerobes examined, no one was indistinguishable. Two environmental isolates were identified as hydrogen-producing bacteria, which were new species in present taxon. Rennanqilyf 3 could not be associated with any Clostridium sp. studied. Rennanqilyf 1 could be classified into Clostridium genus. The combination between 16S rDNA equencing and length polymorphisms of IRS in 16S-23S rDNA is a better method for determining species of the hydrogen-producing bacteria.
【Fund】: Sponsored by Program of Shanghai Education Committee (Grant No07ZZ156);; Key Subject Construction of Shanghai Education Committee(Grant NoP1402);; the National Natural Science Fund of China(Grant No30470054)
【CateGory Index】: X506
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 Ren Nanqi 1,Lin Ming 1,Ma Xiping 2,Wang Aijie 1,Li Jianzhen 1 (1. School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China; 2. Department of Environmental Science, Liaoning University, Shenyang 110036, China);A STRAIN OF ANAEROBIC BACTERIA SCREENED FOR HIGH EFFICIENT HYDROGEN PRODUCTION AND ITS ACIDURIC CHARACTER[J];Acta Energiae Solaris Sinica;2003-01
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Jian-fu1, WANG Ping1, LI Han-dong2, LIU Qin3,LI Chang-zheng3(1 School of Life Science and Technology, Central South Forestry University, Changsha 410004, China; 2 Chinese Research Academy of Environmental Science, Beijing 100012, China; 3 School of Resource and Environmental Science, Wuhan University, Wuhan 430072, China );Research and application of dominant bacteria in treatment of alcohol wastewater[J];Journal of Safety and Environment;2006-02
2 LIU Bin,WU Ke,CAI Jing-min,LI Shao-hui,YU Zhi-min,JIN Jie(Department of Biological and Environmental Engineering,Hefei University,Hefei 230022,China);Study on the Hydrogen Production from Brewer Waste Water[J];Packaging and Food Machinery;2006-01
3 CHEN Ying1,2,REN Nan-qi 1,LI Yong-feng1,ZHANG Da-wei2,CHENG Yao1(1.School of Municipal and Environmental Engineering,Harbin Institute of Technology; 2.Department of Biology,Harbin Normal University,Harbin150090,China);Identification and comparison of biology characteristics among three novel fermentative hydrogen producing bacteria[J];Industrial Microbiology;2007-06
4 LI Yong-feng, REN Nan-qi, CHEN Ying, LI Jian-zheng, HU Li-jie, ZHENG Guo-xiang(School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China);The experimental anaerobic operation of isolation and culture on hydrogen-producing and fermentative bacteria[J];Journal of Harbin Institute of Technology;2004-12
5 LU Wenyu,WEN Jianping,CHEN Yu,LIU Minghui(School of Chemical Engineering & Technology,Tianjin University,Tianjin 300072);Screening of hydrogen-producing aciduric Enterobacter aerogenes strain using He-Ne laser irradiation[J];Chemical Industry and Engineering Progress;2006-07
6 QIN Zhi~(1,2),REN Nan-qi~2,LI Jian-zheng~2(1.School of Chemical and Environmental Engineering,Harbin University of Science and Technology,Harbin 150040,China;2.School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China);Bioaugmentation of Hydrogen Producing Bacteria on Operation of Bio-hydrogen Producing Reactor[J];Environmental Science;2007-12
7 Zhao Dan1,Yu Ruizhang1,Li Xiuyan1,Niu Dongjie2,Zhao Youcai2.(1.Department of Environmental Science and Technology,East China Normal University,Shanghai 200062;2.The State Key Laboratory of Pollution Control and Resource Reuse,Tongji University,Shanghai 200092);A brief look at research on anaerobic dark fermentative hydrogen-producing bacteria[J];Environmental Pollution & Control;2009-01
8 LI Yong-feng, ZHENG Guo-xiang, HU Li-jie, ZHANG Wen-qi,LI Jian-zheng (School of Municipal and Environmental Engineering,Harbin Institute of Technology, Harbin 150090,China);Study on judging hydrogen-producing bacteria by gas chromatography analysis of acidic end products[J];Journal of Harbin University of Commerce(Sciences Edition);2005-01
9 LI Yong-feng, ZHANG Wen-qi ,LIU Da-wei, ZHENG Guo-xiang ,LI Jian-zheng, CHEN Ying (School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China;Heilongjiang,Hegiene School, Harbin 150036,China);Isolation and characterization of several fermentative H_2-producing bateria in hydrogen production system by actvitaed sludge[J];Journal of Harbin University of Commerce(Sciences Edition);2005-05
10 CHEN Ying1,2,REN Nanqi1,CHENG Yao1,LI Yongfeng1,ZHANG Dawei2(1.School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China;2.Department of Biology,Harbin Normal University,Harbin 150080,China);Isolation of Three Novel Fermentative H2-Producing Bacteria and Comparison of Their H2-Producing Ability under Different Conditions[J];Bulletin of Science and Technology;2008-01
China Proceedings of conference Full-text Database 3 Hits
1 ;The Improvement of Anaerobic Operation and Isolation Culture on Hydrogen-producing and Fermentative Bacteria[A];[C];2003
2 ;The Genes Of Hydrogen-producing in Anaerobic Bacterium and Its Cloning Strategy[A];[C];2003
3 M Lin, N Q Ren, D T Liang, A J Wang, J H Tay (School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin 150090, P R China; Institute of Environmental Science & Engineering, Nanyang Technological University, Singapore 637723, Singapore);Bio - hydrogen Porduction[A];[C];2003
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