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《哈尔滨工业大学学报(英文版)》 2007-05
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Length polymorphisms for intergenic spacer regions of 16S-23S rDNA in members of the new hydrogen-producing bacteria

LI Yong-feng1,2,3, XU Jing-li1, YANG Chuan-ping2, REN Nan-qi3 (1.Chemical Engineering School, Shanghai University of Engineering and Sciences, Shanghai 201620, China, 2.Forestry School, Northeast Forestry University, Harbin 150040, China; 3.Municipal and Environmental Engineering School,Harbin Institute of Technology, Harbin 150090,China)  
A method based on PCR amplification of the 16S rRNA gene (rDNA)-23S rDNA intergenic spacer regions (ISR) was developed for the identification of species within the novel group hydrogen-producing anaerobes. The sizes of the PCR products varied from 1264 to 398 bp. Strain of isolate Rennanqilyf 3 was characterized as having products of 1262,398,638,437 and 436 bp. The isolate Rennanqilyf 1 had product of 1264 bp. The isolate Rennanqilyf 13 had products of 1261,579 and 485 bp. Of the 3 species of the novel group hydrogen-producing anaerobes examined, no one was indistinguishable. Two environmental isolates were identified as hydrogen-producing bacteria, which were new species in present taxon. Rennanqilyf 3 could not be associated with any Clostridium sp. studied. Rennanqilyf 1 could be classified into Clostridium genus. The combination between 16S rDNA equencing and length polymorphisms of IRS in 16S-23S rDNA is a better method for determining species of the hydrogen-producing bacteria.
【Fund】: Sponsored by Program of Shanghai Education Committee (Grant No07ZZ156);; Key Subject Construction of Shanghai Education Committee(Grant NoP1402);; the National Natural Science Fund of China(Grant No30470054)
【CateGory Index】: X506
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