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Isolation,Identification and Characters of Toluene Degrading Bacteria

Huo Danqun~1,Sun Xingfu~1,Rao Jiajia~1,Hou Changjun~2,Liao Qiang~3(1.Key Laboratory of Biomechanics and Tissue Engineering under the State Education Ministry,College of Bioengineering,Chongqing University,Chongqing 400044,China;2.College of Chemistry and Engineering,Chongqing University,Chongqing 400044,China;3.Institute of Engineering Thermophysics,Chongqing University,Chongqing 400044,China)  
A Gram negative bacterium capable of utilizing toluene as sole carbon and energy sources was isolated from active sludge in the dephenolizing pool workshop of coking plant of Chongqing Iron and Steel Group Co.Ltd..It was identified primarily as Pseudomonas sp.based on the physiological and biochemical experiment results,colony characteristics and mycelial morphology.The optimum growth conditions of the strain are pH 7.0,temperature 35℃,60μL toluene in 100mL medium and inoculum 5%.The toluene-degrading characteristics of the strain were investigated.When the mass concentration of toluene is 150mg/L,the specific degradation rate is 0.211h~(-1).
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