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《Journal of Chemical Industry and Engineering(China)》 2000-S1
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CHROMATOGRAPHIC PROTEIN REFOLDING

Gu Zhenyu and Su Zhiguo (State Key Laboratory of Biochemical Engineering, Instigate of Chemical Metallurgy, Chinese Academy of Sciences, Beijing 100080)  
Protein refolding is a bottle-neck for large scale production of valuable proteins expressed as inclusion body in E.coli. The trends and advantages of applying chromatography in protein renaturation process are investigated with regard to suppressing aggregation and enhancing refolding. A new process of urea gradient gel filtration refolding is discussed in comparison with the traditional gel filtration process.
【CateGory Index】: O629
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