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《Journal of Chemical Industry and Engineering(China)》 2000-S1
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Gu Zhenyu and Su Zhiguo (State Key Laboratory of Biochemical Engineering, Instigate of Chemical Metallurgy, Chinese Academy of Sciences, Beijing 100080)  
Protein refolding is a bottle-neck for large scale production of valuable proteins expressed as inclusion body in E.coli. The trends and advantages of applying chromatography in protein renaturation process are investigated with regard to suppressing aggregation and enhancing refolding. A new process of urea gradient gel filtration refolding is discussed in comparison with the traditional gel filtration process.
【CateGory Index】: O629
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Chinese Journal Full-text Database 9 Hits
1 LI Lin, DONG Xiaoyan and SUN Yan (Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China);DYNAMIC FED-BATCH REFOLDING BEHAVIOR OF DENATURED-REDUCED LYSOZYME AT HIGH CONCENTRATIONS[J];Journal of Chemical Industry and Engineering(China);2003-12
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3 ZHANG Shou-tao1,2, SHI Jing1, GUO Ai-guang1 (1. Key Lab. Agricultural Molecular Biology in Shaanxi Province, Northwest A & F University. Yangling, Shaanxi 712100, China; 2. Department of Bioengineering, Zhengzhou University, Zhengzhou, Henan 450001, China);Refolding of Recombinant Snake Venoms Fibrino(geno)lytic Emzyme Fibrolase[J];The Chinese Journal of Process Engineering;2007-04
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7 DOU Jia CAI He JI Fang-ling CUI Wen-ju WANG Jing-yun BAO Yong-ming AN Li-jia(Department of Bioscience & Biotechnology,Dalian University of Technology,Dalian 116024,China);Site-directed Mutagenesis and Enzymatic Activity Assay of Gln49-Phospholipase A_2 Mutant[J];China Biotechnology;2007-05
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9 XU Qiu-xiang1, LIU Na1, LIU Liao1, SHI Zhen-sheng1, LIU Wen-jun2 (1.College of Animal Medicine, China Agriculture University, Beijing 100094, China 2.Chinese Academy of Science Institute of Microbiology, Beijing 100080, China);Cloning and Prokaryotic Expression and Activity measurement of the IFNω Gene of feline[J];Chinese Journal of Veterinary Medicine;2007-09
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