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《Journal of Chemical Industry and Engineering(China)》 2002-12
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OXYGEN-SUPPLY CONTROL MODE FOR EFFICIENT PRODUCTION OF PYRUVATE IN BATCH PROCESS

LI Yin, CHEN Jian and LUN Shiyi(Key Laboratory of Industrial Biotechnology, Ministry of Education , Southern Yangtze University, Wuxi 214036, Jiangsu , China)  
The effect of volumetric oxygen transfer coefficient (kLa), on the performance of producing pyruvate by a multi - vitamin - auxotrophic yeast of Torulopsis glabrata , was investigated in batch fermentation process. High yield of pyruvate to glucose (0.797 g·g-1) was achieved at higher kLa (450 h-1), while the glucose consumption rate was low (1.14 g·L-1·h-1). Glucose consumption was enhanced at lower kLa (200 h-1), however, the yield of pyruvate to glucose decreased to 0.483 g·g-1. Based on the characteristics of process parameters and carbon flux distribution, a two - stage oxygen supply control mode, in which kLa was controlled at 450 h in the first 16 h then switched to 200 h after 16 h, was proposed and experimentally verified. Relative high concentration of pyruvate (69.4 g·L-1), high yield of pyruvate to glucose (0.636 g· g-1), and high glucose consumption rate (1.95 g·L-1·h-1) was achieved by applying this mode. The productivity (1.24g·L-1·h-1) was improved by 36% , 23% and 31% than the cases of constant KLa (450 h-1, 300 h-1 and 200 h-1), respectively. Experimental results indicate that the difference between the performance of producing pyruvate under favorable state of oxygen- supply was caused by the different energy state of cell, which may be due to the different regenerated pathways of NADH generated from glycolysis.
【Fund】: 江苏省“九五”工业重大科技攻关项目(No.BG98015-3).
【CateGory Index】: TQ921.7
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