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《Journal of Chemical Industry and Engineering(China)》 2004-07
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WANG Yajun 1, YAO Shanjing 1 and WU Tianxing 2 ( 1Department of Chemical and Biochemical Engineering, 2Department of Chemistry, Zhejiang University, Hangzhou 310027,Zhejiang, China)  
Much attention has been paid to the influences of osmosis on Saccharomyces cerevisiae, due to the increasing application in the food and pharmaceutical industries, biotransformations. Besides, to maintain an appropriate turgor is of crucial importance to individual growth, cellular division and viability.In the study, to evaluate the impacts of different osmolytes on cellular proliferation, morphology and viability of the model microbe Saccharomyces cerevisiae FL 1, in additon to the commonly used sodium chloride, glycerol, poly(ethyleneglycol)600, and sorbitol were used.Moreover, the dynamic behavior of cell proliferation and metabolism under physiologicallg acceptable hyperosmotic condition were examined. Results indicated that these osmolytes had significant negative impacts on proliferation with increasing concentration, compared to the control treatment composed of YEPD media. The cellular morphology was close to sphere after exposure to high osmotic pressure for 24 h. After analyzing the dynamics data, sodium chloride of 0.5 mol·L -1 was found to markedly reduce the specific cell growth rate, sugar consumption but prolong the duration of exponential growth phase. At the same time, PEG600 of 0.33 mol·L -1 had no marked influence on cell proliferation but shortened the stationary phase.However, under the physiologically acceptable hyperosmotic condition, the final biomass density among all the treatments was similar.
【Fund】: 国家自然科学基金资助项目 (No 2 0 2 760 65 )~~
【CateGory Index】: Q933
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