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《Journal of Chemical Industry and Engineering(China)》 2004-07
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INFLUENCES OF OSMOLYTES ON CELLULAR PROLIFERATION, MORPHOLOGY AND VIABILITY OF Saccharomyces cerevisiae

WANG Yajun 1, YAO Shanjing 1 and WU Tianxing 2 ( 1Department of Chemical and Biochemical Engineering, 2Department of Chemistry, Zhejiang University, Hangzhou 310027,Zhejiang, China)  
Much attention has been paid to the influences of osmosis on Saccharomyces cerevisiae, due to the increasing application in the food and pharmaceutical industries, biotransformations. Besides, to maintain an appropriate turgor is of crucial importance to individual growth, cellular division and viability.In the study, to evaluate the impacts of different osmolytes on cellular proliferation, morphology and viability of the model microbe Saccharomyces cerevisiae FL 1, in additon to the commonly used sodium chloride, glycerol, poly(ethyleneglycol)600, and sorbitol were used.Moreover, the dynamic behavior of cell proliferation and metabolism under physiologicallg acceptable hyperosmotic condition were examined. Results indicated that these osmolytes had significant negative impacts on proliferation with increasing concentration, compared to the control treatment composed of YEPD media. The cellular morphology was close to sphere after exposure to high osmotic pressure for 24 h. After analyzing the dynamics data, sodium chloride of 0.5 mol·L -1 was found to markedly reduce the specific cell growth rate, sugar consumption but prolong the duration of exponential growth phase. At the same time, PEG600 of 0.33 mol·L -1 had no marked influence on cell proliferation but shortened the stationary phase.However, under the physiologically acceptable hyperosmotic condition, the final biomass density among all the treatments was similar.
【Fund】: 国家自然科学基金资助项目 (No 2 0 2 760 65 )~~
【CateGory Index】: Q933
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【References】
Chinese Journal Full-text Database 4 Hits
1 ZHAO Shuo et al (School of Life Sciences and Technology,Tongji University,Shanghai 200092);Breeding of High-sugar-tolerant Yeast and Application[J];Journal of Anhui Agricultural Sciences;2010-09
2 WU Shuai, XIAO Dong-guang, YUAN Tong-lei and ZHAO Xu (College of Bioengineering, Tianjin University of Science and Technology, Tianjin, 300222);Selection of Saccharomyces cerevisiae with Good Osmo-, thermo-tolerance and High Ethanol-production Capacity[J];Liquor-Making Science & Technology;2006-09
3 Zhang Lixing~1 Zhang Yinjun~1 Ding Yuyun~2 1(College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, China) 2(Pingyang Bureau of Science and Technology, Pingyang, 325400, China);Brewing Yeast Stresses and Responses during Beer Brewing[J];Food and Fermentation Industries;2005-06
4 Dong Xinfu1,Dong Liang1,Dong Chuanliang2,Miao Jun1,Han Dan1,Zhao Changxin11(Dalian Polytechnic University,School of Bio & Food Technology,Dalian,116034,China) 2(Xiwang Group,Zouping 256209,China);Key of the Secreted Organic Acid of Saccharomyces cerevisiae Under High Concentrition Sugar[J];Food and Fermentation Industries;2010-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Zhang Ming(Department of biological engineering,Anhui Agricultural University,Hefei 230036)Wang Yuanjun;Pan Renru(Department of biology,University of Science&Technology of China,Hefei 230026);An Investigation of the Formation and Regeneration of the Saccharomyces cerevisiae Protoplast[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;1996-01
2 ZHANG Bo, SU Yong ping, AI Guo ping, LIU Xiao hong, WEI Yong jiang, WANG Feng chao, CHENG Tian min (Research Institute of Combined Injury of PLA, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China);Differential proteomic analysis of intestinal epithelium cell line before and after irradiation by gamma-ray[J];Acta Academiae Medicinae Militaris Tertiae;2003-24
3 Chen Zhihua(Fujian Xuejin Beer Group, Putian 351111);Raise the Activity of Yeast with Yeast Nutrients[J];THE LIGHT & TEXTILE INDUSTRIES OF FUJIAN;1999-03
4 MENG Chen1, YE Qin2, SHI Xian-ai1, CHENG Jian-feng1, GUO Yang-hao1, (1. Department of Biotechnology, Fuzhou University, Fujian 50002, China; 2. State Key Laboratory of Bioreactor, East China University of Science and Technology, Shanghai 200237, China);Study on Carbon Metabolic Flux Characteristic of Streptomyces aureofaciens[J];Journal of Chemical Engineering of Chinese Universities;2004-02
5 QIAN Jun-qing,LIN Chun-mian,CHEN Xiao-long,LIN An(College of Biological and Enviromental Engineering,Zhejiang University of Technology,Hangzhou 310014,China);Study on Supercritical CO_2 Influence on Growth of Yeast[J];Journal of Chemical Engineering of Chinese Universities;2004-03
6 Ling Li Chen Guangzhi Zhou Hezhi (College of Biotechnology & Sugar Industry Engineering, Guangxi Univ.,10 Xixiangtanglu,Nanning,Guangxi,530004);Introduction to the Theory and Practice of Metabolic Control Analysis[J];GUANGXI SCIENCES;1999-02
7 MA Li an (Department of Food Science, Hubei Agricultural College,Jingzhou,Hubei 434103,China);Acclimation and Screening of Alcohol Yeast Tolerant to High Temperature[J];JOURNAL OF HUBEI AGRICULTURAL COLLEGE;2000-01
8 GAO Yu-rong, WANG Xia, FENG Chang-you;STUDY OF INCREASING QUALITY OF SOY SAUCE WITH SOY SAUCE DRAFF BY MIXED FERMENTATION[J];JOURNAL OF HEILONGJINAG AUGUST FIRST LAND RECLAMATION UNIVERSITY;2000-03
9 Liu Suchun Zhou Chuanyun Liu Fengyu (College of Foodstuff Science & Technology,HNAU,Changsha,410128);THE SIFTING OF APRICOTINE YEAST STRAINS[J];JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY;1998-05
10 XU Zhong, HAN Yu-jie and ZHANG Hong-wei (Scholl of Food Engineering,Harbin Commercial Univ.,Harbin 150076,China);Effect factors of Liquidation of Potato Starch[J];Chemistry and Adhesion;2004-01
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 WU Xianfeng, SUN Bo, CHI Yujie, XU Ning (Food College, Northeast Agricultural University, Harbin 150030, China);Application of high glucose-tolerant yeast to alcohol production by sweet sorghum stalk juice fermentation[J];Journal of Northeast Agricultural University;2008-11
2 YI Yi,WU Shi-hua(Department of Biochemical Engineering,Guangxi University of Technology,Liuzhou 545006,China);Screening of yeast strains with alcohol-tolerance[J];Journal of Guangxi University of Technology;2008-03
3 XUE Jun-xia,XU Yan-wen,YANG Ying and LIU Yan-lin (College of Enology, Northwest A&F University, Yangling, Shanxi 712100, China);Investigation on the Tolerance of Wild Saccharomyces cerevisiae[J];Liquor-Making Science & Technology;2007-06
4 YU Zhi-min, LUAN Jing, XU Peng, WANG Ji-hua and ZHAO Chang-xin (Liaoning Key Lab of Fermentation Technology, Dalian Polytechnic University, Dalian, Liaoning 116034, China);Research on the Preparation and Regeneration of Protoplast from High-concentration Mash Resistant Saccharomyces cerevisia[J];Liquor-Making Science & Technology;2008-04
5 JIANG Zong-Bao1,2,MIAO Jun1,ZHAO Rui1,LUAN Jing1 and ZHAO Chang-xin1(1.Key Lab of Fermentation Engineering of Liaoning Province,School of Bio & Food Technology,Dalian Polytechnic University,Dalian,Liaoning 116034;2.Huarun Snow Beer Co.Ltd.,Chaoyang,Liaoning 122000,China);Study on Protein Secretion from Saccharomyces Cerevisiae Under High Osmotic Pressure[J];Liquor-Making Science & Technology;2009-05
6 LAN Zhao,TANG Mei-qiong,WEI Ke,MA Guang-ting(Intitute of Applied Microbiology of Agriculture,Guangxi University,Nanning 530005,Guangxi,China);Screening of Respiration-deficient Saccharomyces cerevisiae Strains with Sugar-and Thermo-tolerances[J];Life Science Research;2009-03
7 Chen Hongyun,Ye Yanrui,Zhu Yi,Zheng Suiping,Lin Ying(School of Bioscience and Bioengineering,South China University of Technology,Guangzhou 510006,China);Comparison of Yeast Tolerance Evaluation Methods[J];Food and Fermentation Industries;2008-12
8 Qin weishuai~1,Wang Haiyan~1,Li Chao~2,Lv Peng~1,Zhao Xinjie~(~1*) (1.Food and Bioengineering Department,Shandong Institute of light Industry,Jinan 250533; 2.Longkou Branch,Shandong Agriculure Broadcast & Television School,Shandong Longkou 265701);Comparison of tolerance capacity among several different wine yeasts[J];Sino-Overseas Grapevine & Wine;2009-09
9 WANG Ji-hua1,2, YANG Fang3, ZHAO Chang-xin1* (1.Key Lab. of Fermentation Engineering, Liaoning Province, School of Bio & Food Technology, Dalian Polytechnic University, Dalian 116034; 2.Weinan Duqiao High School, Weinan 714000; 3.Institute of Agriculture Quality Standards & Testing Technique,Yunnan Academy of Agricultural Science, Kunming 650223);Study on the osmotic regulatory mechanism of Saccharomyces cerevisiae under the stress of NaCl[J];Food Science and Technology;2009-11
10 WANG Jihua1,XUE Yongchang1,GAO Sheng2,TANG Jiaxin1,YU Zhimin1,ZHAO Changxin1*(1.Liaoning Key Laboratory of Fermentation engineering,College of Biology & Food Engineering,Dalian Polytechnic University, Dalian 116034,China;2.Liaoning Huarun Snow Beer Dandong Co.Ltd.,Dandong 118001,China);Studies of osmotic stress on yeast physiological functions[J];China Brewing;2008-05
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