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《CIESC Journal》 2012-07
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Activities changes of key enzymes in glutamate fermentation in response of various initial biotin contents

CAO Yan,Enock Mpofu,DING Jian,DUAN Zuoying,SHI Zhongping(Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology, Jiangnan University,Wuxi 214122,Jiangsu,China)  
In glutamate fermentation,initial biotin content in the medium varies with the corn slurry originating sources or even production batches,and this variation affects fermentation performance and stability.In this study,activities changes of the key enzymes at metabolic nodes of pyruvate,isocitrate and α-ketoglutarate when initial biotin content was at normal/improper levels,particularly when initial biotin content was at improper levels but adopting faults-rescue measures during fermentation were carefully investigated.When initial biotin was in shortage,all activities of the key enzymes responsible for catalyzing the main glutamate synthesis route were weakened,in particular,the activity of α-oxoglutamate dehydrogenase complex was inactivated and energy metabolism completely relied on glyoxylate cycle.When biotin in shortage was determined and biotin was supplemented,the enzyme responsible for the central metabolic route,pyruvate dehydrogenase returned to the normal level,and TCA cycle turned to be the main energy metabolism route once again.On the other hand,when initial biotin was in excess,all activities of the key enzymes were activated except pyruvate carboxylase and glutamate dehydrogenase.When biotin in excess was determined and Tween 40 was supplemented,the activities of two key enzymes responsible for glutamate synthesis,pyruvate dehydrogenase and isocitrate dehydrogenase,remained at high levels.At the same time,activities of α-oxoglutamate dehydrogenase complex and isocitrate lysase declined to the normal levels.The final glutamate concentration of the failure-likelihood fermentations could be recovered back to the control level(75—80 g·L-1)by using the relevant rescue measures.Furthermore,final glutamate concentration could be further increased and reach 87 g·L-1,which was over 108.8% of that obtained in the control case,when adopting the "two-stage" optimal operation mode based on combined adjustment of initial biotin content and Tween 40 addition.
【Fund】: 国家高技术研究发展计划项目(2006AA020301-11);; 国家重点基础研究发展计划项目(2007CB714303)~~
【CateGory Index】: TQ922.1
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 CAO Yan* DING Jian DUAN Zuo-Ying LIU Li-Ming SHI Zhong-Ping (Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China);On-line Glucose Concentration Prediction and Control for Improving Glutamate Fermentation Performance[J];Microbiology;2009-10
【Co-citations】
Chinese Journal Full-text Database 6 Hits
1 WU Xin-shi,WANG Nan,PENG Yuan,CHEN Shang-bi,SHEN Qian(School of Chemistry and Chemical Engineering,Tianjin University of Technology,Tianjin 300384,China);Compound mutation of a glutamic acid-producing strain and biological qualities of the mutant[J];Journal of Tianjin University of Technology;2012-01
2 GAO Guang-heng1,ZHOU Wan-li2,ZHU Si-rong2,BI Chun-yuan2, ZHAO Xiao-hua2,SHI Jian-guo2(1.Shandong Provincial Key Laboratory of Biosensors,Biotechnology Center,Shandong Academy of Sciences, Jinan 250014,China;2.Institute of Biology,Shandong Academy of Sciences,Jinan 250014,China);A DNS based reducing sugar detector[J];Shandong Science;2012-03
3 Cao Yan1,Enock Mpofu2,Ding Jian1,Duan Zuo-ying1,Shi Zhong-ping1 1(Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology, Jiangnan University,Wuxi 214122,China) 2(Harare Institute of Technology,Harare,Zimbabwe);Improving Glutamate Yield by Adding NaHCO_3 and Regulating pH in the Glutamate Fermentation[J];Food and Fermentation Industries;2012-12
4 JIN Cheng (The Editorial Board of Microbiology, Beijing 100101, China);On-line Control of Glutamate Fermentation[J];Microbiology;2009-10
5 ZHANG Junli1,2,LIU Long1,2,FANG Jun1,2,LI Jianghua1,2,DU Guocheng1,2,CHEN Jian3,NING Jianfei4 & CAI Liming4(1School of Biotechnology,Jiangnan University,Wuxi 214122,Jiangsu,China)(2Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,Jiangsu,China)(3State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,Jiangsu,China)(4Wuxi Jinghai Amino Acid Co.Ltd.,Wuxi 214199,Jiangsu,China);Optimization of L-isoleucine Fermentation Conditions Based on DO-stat Feeding Culture Strategy by Brevibacterium lactofermentation[J];Chinese Journal of Applied & Environmental Biology;2011-03
6 LU Dongmei,LIU Jianzhong** and MAO Zongwan The Key Laboratory of Gene Engineering of Ministry of Education,State Key Laboratory of Biocontrol,Sun Yat-Sen University,Guangzhou 510275,China;Engineering of Corynebacterium glutamicum to Enhance L-ornithine Production by Gene Knockout and Comparative Proteomic Analysis[J];中国化学工程学报(英文版);2012-04
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 Jia Zongjian Zhang Xianen (Wuhan Institute of Virology, Academia Sinica, Wuhan 430071);On-line Control of Glucose Concentration Based On Enzyme Electrode System[J];Chinese Journal of Biotechnology;1996-03
2 WANG Yan 1,2 SONG Xiang 1 YANG Ping-Ping 1 DUAN Zuo-Ying 1 MAO Zhong-Gui 1* 1(School of Biotechnology, Southern Yangtze University; the Key Laboratory of Industry Biotechnology, Ministry of Education, Wuxi 214036, China) 2(Department of Food Engineering, the Light-industry Institute of Shandong, Jinan 250100,China);Purification and Characterization of Glutamate Dehydrogenase from Corynebacterium glutamicum S_ (9114)[J];Chinese Journal of Biotechnology;2003-06
3 Feng Derong Shang Xueqin Zhou Wanli Zhu Sirong Qiu Weizhong Zhang Dongyan Li Zhihong Sun ShiqingShi Jianguo(Shandong Research Institute of Biology, Jinan);Study on the Model SBA-40 L-Glutamate and Glucose Bifunctional Analyser Used in the Fermentation Process[J];Food and Fermentation Industries;1993-04
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