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《The Chinese Journal of Process Engineering》 2009-01
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Bacterial Population Composition and Succession in Column Bioleaching Process of Low-grade Copper Sulphide Ore

ZOU Ping1,2, ZHOU Xing-long1, ZHANG Wen-bin1, LIN Lian-bing1, ZHANG Lan-lan1(1.Kunming University of Science and Technology, Kunming,Yunnan 650093, China;2.Technology Center, Yunnan Metallurgy Group Co., Ltd., Kunming, Yunnan 650031, China)  
Based on PCR-DGGE analysis, there were 5 bands in DGGE patterns of PCR amplified 16S rDNA(V3~V6) of bacterial samples of different periods in column bioleaching process of calcopyrite corresponding to bacteria which were highly related to Acidithiobacillus ferrooxidan with 98% sequence similarity.During the bioleaching of 63 d, the bacterial population succession took place.The bacterial succession came about among bacterial strains of A.f.Using a 9K solid culture medium, 3 pure strains of mesophiles were isolated, which were random-sampled from the bioleaching pulp of the ore.The sequence analysis of 16S rDNA showed that 3 pure strains were highly related to A.f with 99% sequence similarity.These results indicated that the dominant bacteria in the bioleaching process of low-grade copper sulphide ore consisting mainly of chalcopyrite was A.f.In the early stages of bioleaching, 02 and 05 bands corresponding to bacterial strains of A.f were chosen in the leaching of easily dissolved secondary copper sulphide ore.In the middle and latter stages of bioleaching, 01, 02, 03, 04 and 05 bands corresponding to the bacterial strains of A.f were selected in the leaching of hardly dissolved primary ore.The technical parameters of PCR-DGGE analysis were optimized.
【Fund】: 云南省自然科学基金资助项目(编号:2005E0068M)
【CateGory Index】: Q93
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【Citations】
Chinese Journal Full-text Database 7 Hits
1 Xiang Yongjun(Dexing Copper Mine);Oxidization Effect of Thiobacillus Ferroxidans on Chalcopyrite[J];Metal Mine;2000-10
2 ZHANG Wei qing 1, WEI De zhou 1, SHEN Jun 2 (1. Northesat University, Shenyang 110006, China; 2. Shanghai Auditing Affairs office, Shanghai 200040, China);Oxidizing Action of Thiobacillus Ferrooxidans on Chalcopyrite——A Study[J];MINING AND METALLURGICAL ENGINEERING;1999-03
3 ZHANG Zai hai,WANG Dian zuo,QIU Guan zhou,HU Yue hua (Depariment of Mineral Processing,Central South University of Technology, Changsha,Hu’nan 410083,China);The Bacteriological Fundamental of Bacterial Leaching[J];HYDROMETALLURGY OF CHINA;2000-03
4 LUO Hai Feng, QI Hong Yan, XUE Kai, WANG Xiao Yi, WANG Chuan, ZHANG Hong Xun (Environmental Biotechnology Lab., Research Center for Eco Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China);Influence of application of GC-clamp on study of soil microbial diversity by PCR-DGGE[J];Acta Ecologica Sinica;2003-10
5 Wang Tao Chai Lihong Cui Xiaolong Peng Qian Jiang Chenglin* (Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Insti tute of Microbiology, Yunnan University, Kunming 650091, China);Preliminary Study on Bacterial Diversity in a Hot Spring by Using Culture-independent Approach[J];Acta Microbiologica Sinica;2003-05
6 GONG Man-Li REN Nan-Qi * XING De-Feng (School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China);Application of Denaturing Gradient Gel Electrophoresis and Temperature Gradient Gel Electrophoresis in Microbial Molecular Ecology[J];Acta Microbiologica Sinica;2004-06
7 XING De-feng,REN Nan-qi~*(School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China);Common problems in the analyses of microbial community by denaturing gradient gel electrophoresis(DGGE)[J];Acta Microbiologica Sinica;2006-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 JIANG Jian-wen et al (Pearl River Fisheries Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong 510380);Comparison on The Difference of Microbial Diversity between Artificial Biofilm and Water in Pond by DGGE Technique[J];Journal of Anhui Agricultural Sciences;2008-21
2 YANG Li-li1,YANG Hong-ying2,GONG En-pu1,TONG Lin-lin2(1.School of Resources & Civil Engineering,Northeastern University,Shenyang 110004,China;2.School of Materials & Metallurgy,Northeastern University,Shenyang 110004,China.);Study on the Bio-oxidation Process of Three Single Iron Sulfide Minerals[J];Journal of Northeastern University(Natural Science);2007-06
3 ZHANG Ying~(1,2) YANG Changfu~1 WANG Aijie~3 1 Resources and Environmental Sciences College,Northeast Agricultural University,Harbin 150030,China 2 Department of Civil and Environmental Engineering,University of California,Berkeley CA94703,USA 3 School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China;The key procedures and advancement in microbial communities analysis of DGGE[J];东北农业大学学报(英文版);2007-03
4 CHU Na1),ZHAO Yuanyi1),ZHANG Guangdi1),ZHANG Qin2),CAI Jianhui1),XIONG Qunyao1),LI Dexian1),WANG Jinsheng3),ZHAO Jinyan3)1)Institute of Mineral Resources,Chinese Academy of Geological Science,Beijing,1000372)Institute of Geophysics and Geochemistry,Chinese Academy of Geological Sciences,Langfang,Hebei,1028493)College of Water Science,Beijing Normal University,Beijing,100875;Environmental Trait of Speciations of Heavy Metals in Low-Grade Ore Plot and Soil of the Dawu River Domain in the Dexing Copper Mine,Jiangxi Province[J];Acta Geologica Sinica;2007-05
5 LUO Qing~(1,2), SONG Ya-na~1, ZHENG Wei-wen~1(1. Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China; 2. Colloge of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Research progress in the diversity of nitrogen fixing microorganism in rice paddy soil[J];Fujian Journal of Agricultural Sciences;2005-S1
6 Zhang Zaihai~1, Wang Dianzuo~2, Deng Jiniu~1, Lindaze~1(1.Western Mining Co.Ltd, Xining 81000,China; 2.Beijing Non-ferrous Institution, Beijing 10088,China);Discuss on the Bacterial Transformation and Leaching Mechanism of Chalcopyrite[J];Engineering Science;2005-S1
7 JI Tian-rong1,2,CHEN Zhuang1(1.Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China; 2.College of Aminal Science,South China of Agricultural University,Guangzhou 510640,China);DGGE technology optimization and applicaton in the animal intestinal microboes[J];Guangdong Agricultural Sciences;2008-06
8 Chen Zhu Chen Yuancai (College of Resources Science and Papermaking Engineering,South China University of Technology,Guangzhou,510640);Application of PCR-DGGE in Microbial Ecosystem Analyses in Pulping and Papermaking Effluent Trentment[J];Paper Science & Technology;2006-06
9 MA Ming-chao 1 ,JIANG Xin 1,2 ,LIU Fei 1 ,LI Jun 2 (1.School of Water Resources and Environment;Beijing Key Laboratory of Water Resources& Environmental Engineering,China University of Geosciences,Beijing 100083,China;2 .Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agriculture Science,Beijing 100081,China);DGGE Analysis of 16S rDNA for Ammonium Oxidizing Bacteria in Constructed Rapid Infiltrition System(CRI)[J];Geological Journal of China Universities;2007-04
10 YANG Cui-yun et al(Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai,Shandong 264003);Research Techniques and Progress of Oil-contaminated Soil Microbial Diversity[J];Journal of Anhui Agricultural Sciences;2009-33
China Proceedings of conference Full-text Database 1 Hits
1 Li Rongxin~1 Xie Gang~1,Yang Di~(1,2),Yang Dajin~3,Zhang Haodong~1(1.The Faculty of Materials and Metallurgical Engineering,Kunming University of Science and Technology,Kunming 650093,China; 2.The Department of Computer Science and Technology,Yunnan University of Finance and Economy, Kunming 650034,China;3.The Technique Center of Yunnan Metallurgy Co.,Ltd.,Kunming 650031, China);Study on Bacterial Leaching of Low Grade Zinc Sulphide Ore[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 TONG Xiong 1,SUN Yon gui 2 ( 1Kunming University of Science and Technology,Kunming,Yunnan,China) ( 2Xingjiang Non ferrous Metals and Industrial School,Urumqi,Xingjiang,China);Research on Techniques for Bioleaching of Chalcopyrite[J];MULTIPURPOSE UTILIZATION OF MINERAL RESOURCES;1999-04
2 ZHANG Wei qing 1, WEI De zhou 1, SHEN Jun 2 (1. Northesat University, Shenyang 110006, China; 2. Shanghai Auditing Affairs office, Shanghai 200040, China);Oxidizing Action of Thiobacillus Ferrooxidans on Chalcopyrite——A Study[J];MINING AND METALLURGICAL ENGINEERING;1999-03
3 XING De-Feng~1, REN Nan-Qi~1*, SONG Ye-Ying~2, LI Qiu-Bo~1,ZHAO Li-Hua~3, XU Xiang-Ling~2 (1. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090;2.College of Life and Environmental Science, Harbin Normal University, Harbin 150080;3. College of Life Science, Heilongjiang University, Harbin 150080).;Application of DG-DGGE to analyze microbial community diversity and population dynamics in fermentative hydrogen-producing system[J];Acta Ecologica Sinica;2005-07
4 GONG Man-Li REN Nan-Qi * XING De-Feng (School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China);Application of Denaturing Gradient Gel Electrophoresis and Temperature Gradient Gel Electrophoresis in Microbial Molecular Ecology[J];Acta Microbiologica Sinica;2004-06
5 TONG Xiong, GUO Xuejun, HUANG Qingqi, QIAN Xin (Department of Resource Exploiting, Kunming University of Science and Technology, Kunming\ 650093);RESEARCH ON MECHANISM OF BIOLEACHING SULFIDE BY ACTIVE ENERGY[J];NONFERROUS METALS;1998-02
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