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THE DETERMINATION OF ENZYMIC ACTIVITY AND ITS INHIBITION ON CATALASE BY ULTRAVIOLET SPECTROPHOTOMETRY

Xu Jingbo Yuan Xiaofan Lang Peizhen(Department of Environmental Science, Northeast Normal University, Changchun, 130024)  
Liver catalase (Ct) activities of carp were measured by ultraviolet spectropho-tometry and the results were reliable. The effect of p-DNB on Ct of carp liver were studied in Vivo. The results showed that p-DNB exhibited fairly strong inhibition to Ct of carp livers. A satisfactory straight line was obtained by plotting the probits of inhibition percentage of Ct against the logarithmic concentration of toxicants, The concentration causing 50% inhibtion of p-DNB to Ct carp liver was 0. 087mg·I-1. It was proposed that the effect of toxicant on Ct could be used as an index of ecotoxicolgical assessment.
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