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《Chinese Journal of Environmental Engineering》 2014-08
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Effect of sources on screening and degradation ability of PAHs-degrading bacteria

Wu Fengji;Zhang Menglu;Guo Chuling;Liao Changjun;Chen Meiqin;Dang Zhi;College of Environmental and Energy,South China University of Technology;The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters,Ministry of Education;Guangdong University of Petrochemical Technology;  
It is important to screen highly efficient degrading bacteria for bioremediation of PAHs-contaminated environment. 14 PAH-degrading bacterial strains,which can use pyrene and PAHs( phenanthrene,fluoranthene and pyrene) as sole carbon source,were isolated from oil-contaminated soil and activated sludge of coking wastewater,respectively. The results showed that the isolated bacterial strains belonged to 9 genera,Mycobacterium sp.,Ralstonia sp. and Shinella sp. were the three common genera from both enrichment sources. The isolated bacterial strains( CP16 and CM32) with the highest degrading abilities were isolated from activated sludge of coking wastewater. Both bacterial strains were identified as Mycobacterium spp. After 7-day incubation,degradation percentage of pyrene(50 mg /L) by bacterial strain CP16 was 74. 99%,while degradation percentage of mixed PAHs( phenanthrene 50 mg /L,fluoranthene and pyrene,each of 10 mg /L) by strain CM32 reached 100%. Therefore,activated sludge of coking wastewater has the advantage to screen higher-efficiency PAHs-degrading bacteria,when it is used as a screening source.
【Fund】: 国家“863”高技术研究发展计划项目(2012AA101403);; 广州市科技计划项目(12C62081569);; 广州市珠江科技新星项目(2011J2200064);; 广东省自然科学基金研究团队项目(9351064101000001)
【CateGory Index】: X172
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