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《Environmental Science》 2005-04
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Microbial Community Structure Analyzed by Single-Strand Conformation Polymorphism Technique in Sulfate-Reducing Reactor

ZHAO Yang-guo, REN Nan-qi, WANG Ai-jie, LIU Guang-min, ZHAO Qiu-shi, SHANG Huai-xiang (School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China)  
Analyses of microbial community structure and the relationships between Sulfate-Reducing Bacteria (SRBs) and Acidogenic Bacteria (ABs) in a completely stirred sulfate-reducing reactor were carried out by modified polymerase chain reaction-single-stranded conformation polymorphism (PCR-SSCP) targeted eubacterial 16S ribosomal RNA gene. A total of 13 bands were obtained and 6 of them (A1, A3, A4, A5, A9, A10) were sequenced. The sequences are similar to Leuconostoc mesenteroides (GenBank Access No.AY453065), some uncultured bacteria (AJ318147, AF227834, AJ576427), Ethanologenbacterium (AY434722), Clostridiaceae (AB084627), etc. In order to investigate the SRBs in the reactor,the active sludge was cultured on SRB-selected media and also did SSCP with the compound cultured bacteria. Two new bands appeared, one similar to Bacteroidetes (AB074606) and another similar to Desulfovibrio (Y12254, U42221). The experimental results indicate that the proportion of SRBs in the reactor is probably less than 1.5 percent. But the few SRBs play a very important role during the course of sulfate reduction via cooperation with acidogenic bacteria.
【Fund】: 国家自然科学基金资助项目(50208006)
【CateGory Index】: X172
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