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《Environmental Science》 2007-04
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Microbial Community Structure in Bio-Ceramics and Biological Activated Carbon Analyzed by PCR-SSCP Technique

LIU Xiao-lin,LIU Wen-jun(Department of Environmental Science and Engineering,Tsinghua University,Beijing 100084,China)  
Analyses of microbial community structure in bio-ceramics(BC) and biological activated carbon(BAC),which widely used in drinking water treatment were performed by polymerase-chain-reaction-single-strand-conformation-polymorphism (PCR-SSCP) targeted eubacterial 16S ribosomal RNA gene.Microorganisms on bio-ceramics and biological activated carbon were detached by ultrasonic,culturing on R2A and LB agar,respectively,followed by genome DNA extracting.Results show that larger than 10 kb genome DNA could be extracted from all the samples except the BAC samples processed by ultrasonic.However,quantities of the extracted DNA were different.408 bp gene fragments were observed after PCR using the extracted genome DNA as templates.These gene fragments were digested with lambda exonuclease followed by SSCP electrophoresis.Same SSCP profiles were observed between ultrasonic eluting,R2A and LB agar culturing.The identity of the segment from bio-ceramics with uncultured Pseudomonas sp.Clone FTL201 16S rDNA(GenBank,AF509293.1)fragment was 92%,and identities of the two segments from BAC with Bacillus sp.JH19 16S rDNA(GenBank,DQ232748.1)fragment and Bacterium VA-S-11 16S rDNA(GenBank,AY395279.1)fragment were 100% and 99%,respectively.
【Fund】: 全国优秀博士论文基金项目(200243)
【CateGory Index】: TU991
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Chinese Journal Full-text Database 3 Hits
1 WANG Yan1,SHEN Xi-quan2,WU Zu-fang1,2,WENG Pei-fang2(1.Key Laboratory of Applied Marine Biotechnology,Ministry of Education;2.Faculty of Life Science and Biotechnology,Ningbo University,Ningbo 315211,China);Application of PCR-SSCP Technology in Diversity Analysis of Microbial Communities[J];Biotechnology;2009-03
2 LUO Hui-Bo HUANG Zhi-Guo LI Hao ZHANG Qiang WEI Chun-Hui(Liquor-making Biotechnology & Application Key Laboratory of Sichuan Province,Sichuan University of Science & Engineering,Zigong,Sichuan 643000,China);PCR-SSCP Analysis of the Pronucleus Microbial Communities in Luzhou-flavor Daqu[J];Microbiology;2009-09
3 LUO Huibo,HUANG Zhiguo,LI Hao,ZHANG Qiang,SHEN Caiping(Sichuan Key Laboratory of Liquor-making Biotechnology & Application,Sichuan University of Science & Engineering,Zigong 643000,China);PCR-SSCPanalysis of the eukaryotic microbial communities in Luzhou-flavor Daqu[J];China Brewing;2009-08
Chinese Journal Full-text Database 1 Hits
1 YI Mei-qin,WANG Kai-yun,JIANG Xing-yin,WANG Huai-xun (Department of Plant Protection,Shandong Agricultural Unversity,Taian 271018,China;);ADVANCES OF RESEARCH ON DEGRADATION OF PESTICIDES BY MICROORGANISMS[J];Journal of Shandong Agricultural University;2002-04
Chinese Journal Full-text Database 10 Hits
1 LI Chang-chun et al (Institute of Plant Protection,Anhui Academy of Agricultural Sciences,Hefei,Anhui 230031);Preliminary Study on the Degradation of Microorganism on 2 Kinds of Pesticides[J];Journal of Anhui Agricultural Sciences;2007-35
2 YANG Sheng-ke, DENG Xiao-ni, FENG Xiu-fang,ZHANG Jiang-jiang(School of Environmental Science and Engineering, Chang'an University, Xi'an 710054, China);Adsorption activity of hexachlorocyclohexance on organic modified meerschaum[J];Journal of Safety and Environment;2007-03
3 YAN Chun-xiu1,ZHAO Chang-shan1,LIU Ya-guang1,2(1.Agronomy College,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC;2.Postdoctoral Fluxion Station of Environment Science and Engineering,Harbin Institute of Technology,Harbin Heilongjiang 150001,PRC);Research advances on long residual herbicide degradation by microorganism[J];Journal of Northeast Agricultural University;2005-05
4 ZHANG Guang-zhi1,ZHANG Xin-jian1,HU Jin-dong1,WANG Yi-lian1,YANG He-tong1,2,3(1.SINO-Japanese Friendship Biotechnology Research Center of Shandong Academy of Sciences,Shandong Provincial Key Lab for Applied Microbiology,Jinan 250014,China;2.Institute of Biology,Shandong Academy of Sciences,Jinan 250014,China; 3.College of Life Science,Shandong University of Technology,Zibo 255049,China);Screening and Bioactivities of Bacteria Degrading Organophosphorus Pesticides[J];Journal of Henan Agricultural Sciences;2009-03
5 LI Ning~1, CHEN Jing-ying~2, LI Li~1, LI Ming-shi~3, ZHAO Xiao-song~1 (1. College of Resource and Environmental Science, Jilin Agricultural University, Changchun 130118, China; (2. Department) of Civil and Environmental Engineering, Donghua Institute of Technology, Fuzhou 344000, China; 3. Department of Jilin Science and Technology, Changchun 130041, China);Isolation and Selection of Strains Used to Degrade Organic PCNB Pesticides and Their Application Effects[J];Journal of Jilin Agricultural University;2005-02
6 WEI Ya-hong, ZHANG Xiao-yan, QU Dong (College of Resources and Environment, Northwest A&F University, Yangling 712100, China);Isolation of Bacteria for Degradation of Herbicide 2, 4-D and Their Biological Characteristics[J];Journal of Agro-Environment Science;2007-06
7 WU Hui-ming~1,ZHANG Jing~2,ZHU Guo-nian~(1*)(1.Institute of Pesticide and Environmental Toxicology,Zhejiang University,Hangzhou 310029,China; 2.Zhejiang Center for Disease Prevention and Control,Hangzhou 310009,China);Screening and Indentification of Dominant Degradation Microorganisms to Miticide F1050[J];Chinese Journal of Pesticide Science;2004-02
8 Li Yang,Wang Yuling,Li Jingmiao(Environmental Management College of China,Qinhuangdao Hebei 066004);Organic Pesticides Contamination Status and Countermeasures in China[J];Journal of Environmental Management College of China;2009-03
9 HOU Xianwen(Environment and Plant Protection Research Institute,Chinese Academy of Tropical Agriculture Sciences,Danzhou,Hainan,571737);Biological Remediation Technology for Agrochemicals-contaminated Soils[J];Chinese Journal of Tropical Agriculture;2009-02
10 XIAO Yan-song1,TAN Lin2,CHEN Gui-hua3,LIU Xiang-ying3 (1. Anren Tobacco Company,Anren,423600,China;2. College of Resource and Enviroment,Hunan Agriculture University,Changsha 410128,China;3. College of Bio-Safety Science and Technology,Hunan Agriculture University,Changsha 410128,China);Microbial Degradation of the Sulfonylurea Herbicides[J];Agrochemicals Research & Application;2007-03
Chinese Journal Full-text Database 10 Hits
1 Dong Chengliang Deng Changyan (The College of Animal Science and Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070);Progress of Examination Techniques for Gene Mutation[J];JOURNAL OF YELLOW CATTLE SCIENCE;1998-03
2 ZHAO Yang-guo, REN Nan-qi, WANG Ai-jie, LIU Guang-min, ZHAO Qiu-shi, SHANG Huai-xiang (School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China);Microbial Community Structure Analyzed by Single-Strand Conformation Polymorphism Technique in Sulfate-Reducing Reactor[J];Environmental Science;2005-04
3 ZHAO Yang-guo,WANG Ai-jie,REN Nan-qi,ZHAO Yan(School of Environmental and Municipal Engineering,Harbin Institute of Technology,Harbin 150090,China);Microbial Community Structure in Different Wastewater Treatment Processes Characterized by Single-Strand-Conformation Polymorphism(SSCP) Technique[J];Environmental Science;2006-07
4 MI Wen-xiu,XIE Bing,XU Ya-tong(Tiantong National Station of Forest Ecosystem,Key Laboratory of Urbanization and Ecological Restoration of Shanghai,Department of Environment Science,East China Normal University,Shanghai 200062,China);Analysis of Microbial Community in a Deodorization Biofilter by PCR-SSCP Method[J];Environmental Science;2008-07
5 SHANG Li-qin1,BAI Lin*,TAN Bing,LIU Xiu-fei,ZHANG Hong-tao(College of Animal Science and Technology,Sichuan Agricultural University,Ya 'an Sichuan 625014);Study on Microbial Community Structure in Earthworms Faecal by Single Strand Conformation Polymorphism(SSCP) Technique[J];Acta Ecologiae Animalis Domastici;2008-02
6 ZHANG Wen-xue~1, QIAO Zong-wei~1, XIANG Wen-liang~1, ZHANG Li-ying~1 HU Cheng~2, WANG Zhong-yan~2, HU Yong-song~2 (1.Department of Food Engineering, Sichuan University, Chengdu 610065,China;2.Department of Bioengineering, Sichuan University, Chengdu 610064,China);Micro-ecological Research Progress of the Cellar of Chinese Flavor Liquor[J];Liquor-making;2004-02
7 XIE Bing,DONG Liang (Department of Environmental Science,East China Normal University,Shanghai 200062,China);Study on Microbial Community Structure of Reed Constructed Wetland Using PCR-SSCP[J];System Sciences and Comprehensive Studies in Agriculture;2007-02
8 ZHANG Wen-xue~1,QIAO Zong-wei~1,HU Cheng~2,WANG Zhong-yan~2(1.Dept.of Food Eng.,Sichuan Univ.,Chengdu 610065,China;2.Dept.of Bioeng.,Sichuan Univ.,Chengdu 610064,China);Fungal Diversity of Fermented Grains During the Production of Chinese Strong Aromatic Spirits[J];Journal of Sichuan University(Engineering Science Edition);2006-05
9 ZHANG Hong-Xun, WANG Xiao-Yi, QI Hong-Yan (Environmental Biotechnology Lab., Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China).;Development in research methods of microbial ecology[J];Acta Ecologica Sinica;2003-05
10 Li Yumei1 Yao Jiyuan2 Wu Jing2 Bai Xiujuan1(1Northeast Agriculture University Heilongjiang,Harbin 150030;2Jilin Agriculture University,Changchun 130118);Research and Application Development of PCR-SSCP Technology[J];Biotechnology Bulletin;2007-06
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Liu Qingyu Wang Shuo Zhu Limin Wang Zhang Gao Kong (Environmental Science Department, Nankai University, Tianfin, 300071)Long Yunxian(The Institute of Elementary Organic Chemistry, Nankai University, Tianjin, 300071);THE STUDY ON THE BIODEGRADATION OF NANKAI-PYRETHRIN BY AEROBE[J];Environmental Chemistry;1992-06
2 Yu Yunlong; Fan Defang; Chen Hexing;(Department of Plant Protection , Zhejiang Agric. Univ.);THE CURRENT STITUATION AND RESEARCHTREND OF DEGRADATION OF PESTICIDES.BY MICROORGASISMS[J];;1996-03
3 ZhengZhong (Zhejiang Agricultural University, Han-zhou);Microbial Degradation of Pesticides.[J];Environmental Science;1990-02
4 Yu Yunlong,Sheng Guoyin,Fu Jiamo(State Key Lab of Organic Geochemistry & Guangdong Key Lab of Environ.Protection and Resources Utilization,Guangzhou Institute of Geochemistry,Chinese Academy=of Sciences,Guangzhou 510640),Chen He xing,Fan Defang(Depar;Degradation of Fenvalerate by Microorganism and Its Enzyme.[J];CHINESE JOURNAL OF ENVIROMENTAL SCIENCE;1997-02
5 Wang Yinshan, Pang Xuejun (Wuhan Institute of Virology, Academia Sinica);ISOLATION OF FLAVOBACTERIUM SP. P3-2 AND ITS PROPERTIES RELEVANT TO DEGRADATION OF ORGANOPHOSPHORUS INSECTICIDES[J];Acta Scientiae Circumstantiae;1985-03
6 Shi Guohan (Research Center for Eco-environmental Sciences, Academia Sinica, Beijing);METABOLISM OF MIEYOUNIAOSANHAO BY SOIL MICROORGANISMS[J];Acta Scientiae Circumstantiae;1990-03
7 (Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080);MICROBIAL DEGRADATION METABOLISM OF N-2,4DIMETHYLPHENYL-N′-METHYLFORMAMIDINE(DMA)[J];ACTA SCIENTIAE CIRCUMSTANTIAE;1998-03
8 Shen Biao et al (Department of Microbiology,Nanjing Agricultural University, NANJING 210095);LuxAB-Labeling of Methylparathion-Degrading Bacterium Pseudomonas sp.DLL-1 and Its Fate in the Soil.[J];Rural Eco-environment;2002-01
9 Yu Yunlong Shi Feng Fan Defang(Department of Plant Protection, Zhejiang University, Hangzhou 310029) Sheng Guoyin Fu Jiamo(Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640) [WT5”HZ];DEGRADATION CHARACTERISTICS OF AN IMMOBILIZED ENZYME FOR FENVALERATE[J];Chinese Journal of Pesticide Science;1999-01
10 Yi Meiqin Wang Kaiyun Jiang Xingyin Wang Yuejun(Department of Plant Protection, Shandong Agricultural University,Taian 271018);Studies on the Isolation and Characterization of Fungi for Degradation of Methyl Parathion[J];Chinese Journal of Pesticide Science;2000-04
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