Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Environmental Science》 2011-08
Add to Favorite Get Latest Update

Development of Colloidal Gold Immunochromatographic Strip for Rapid Detection of Domoic Acid

GAO Li-li,CHENG Jin-ping,LIU Yuan-yuan,WANG Qian,WANG Wen-hua(School of Environmental Science and Engineering,Shanghai Jiao Tong University,Shanghai 200240,China)  
The colloidal gold immunochromatographic test strip was developed in order to establish a rapid detection assay of Domoic acid(DA) content in marine shellfish.The colloidal gold with particle diameter 20 nm was obtained by reducing gold chloride with sodium citrate.After identification by electron micrograph,optimum conditions for labeling were determined and colloidal gold was labeled by DA monoclonal antibody.The gold-labeled antibody was coated on some chosen glass fiber and dried.The coating antigen(DA-BSA) and Goat anti Mouse IgG were spotted respectively on a piece of nitrate fiber membrane as test line and control line.Finally the test strips were constructed and the detection sensitivity was measured.The results showed that,the detection limit of colloidal gold immunochromatographic test strip was 20 ng/mL and the whole analysis process could be completed within 15 min.The method established is sensitive and the procedure of determination is simple and quick without special equipment.The colloidal gold immunochromatographic test strip could be widely used for batch detection of domoic acid in shellfish on site and has great prospect for commercial development.
【Fund】: 海洋赤潮灾害立体监测技术与应用国家海洋局重点实验室项目(MATHAB200917)
【CateGory Index】: X830
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Ren-yan1,LIANG Yu-bo1,CHEN Yuan2,XU Dao-yan1,CHEN Bing-jun1,LIU Yong-jian1(1.National Marine Environmental Monitoring Center,Dalian 116023;2.Zodolabs Biotech CO.,LTD.,Nanchang 330029);Development of Gold Immunochromatography Assay for the Detection of Okadaic Acid[J];Journal of Analytical Science;2010-01
2 XI Hai-yan,CAI Qiang,HE Miao,SHI Han-chang (State Key Joint Laboratory of Environmental Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China);Detection of Escherichia coli in Wastewater Based on Enzyme Immunoassay[J];Environmental Science;2005-05
3 LIU Ting-feng,LIU Ya-zi,SUN Cheng~*(State Key Laboratory of Pollution Control and Resource Reuse,Nanjing University,Nanjing 210093,China);Indirect ELISA for the Pyrethroids-Permethrin Residue Detection[J];Environmental Science;2006-02
4 SHENG Jian-wu,HE Miao,YU Shao-qing,SHI Han-chang,QIAN Yi(ESPC State Key Joint Laboratory,Department of Environment Science and Engineering,Tsinghua University,Beijing 100084,China);Detection of Microcystin-LR in Waters Using Indirect Competitive ELISA[J];Environmental Science;2006-06
5 GAO Li-li,LIU Yuan-yuan,CHENG Jin-ping,WANG Qian,WANG Wen-hua (Department of Environmental Science and Engineering,Shanghai Jiao Tong University,Shanghai 200240,China);Preparation of hybridoma cell strains secreting monoclonal antibodies against domic acid[J];Marine Fisheries;2010-03
6 GU Jia-ping, YUAN Tao (School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China);A critical review for determination methods of the marine algal toxin-domoic acid[J];Marine Science Bulletin;2010-04
7 WEI Feng 1, CHENG Fang 2, GONG Jing hong 1, TANG Shou ting 1 (1 Liaoning Entry Exit Inspection and Quarantine Bureau of China, Dalian 116001, China; 2 Dalian University of Technology, Dalian 116012, China);High Performance Liquid Chromatographic Determination of Domoic Acid in Shellfish Samples[J];Chinese Journal of Chromatography;2001-03
8 Wu Duo-jia and Li Feng-qi Institute for Nutrition and Food Safety, China CDC, Beijing 100021, China;Domoic acid and human health[J];Journal of Hygiene Research;2005-03
9 LIU Renyan,XU Daoyan,DONG Yuhua,LIANG Yubo National Marine Environmental Monitoring Center,Dalian 116023,China;A indirect competitive ELISA to detect domoic acid in seawater and shellfish[J];Journal of Hygiene Research;2009-05
10 CHEN Xiao-feng, LIU Shu-zhao(Department of Plant Protection, Yangzhou University, Yangzhou 225009, C hina);Colloidal Gold Lebelling Immunoassay and It's Application in Rapid Det ection of Small Molecule[J];Pharmaceutical Biotechnology;2004-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Jinga,WANG Chun-na b,WEI Chao-junb,ZHAO Jian-zhuangb,SHI Ya-juna (a.Department of Plant Science and Technolog,b.Department of Basic Sciences,Beijing University of Agriculture,Beijing 102206,China);Development of ELISA Kit for Deltamethrin Residue[J];Journal of Beijing University of Agriculture;2009-02
2 XUAN Guangshan*,LI Xiangpeng,PAN Shijia(Department of Pharmacy,Qingdao University of Science & Technology,Qingdao Shandong 266042,China);Thiophene Boronic Acid Modified Gold Nanoparticle Fluorescence Detection of Glucose[J];Chinese Journal of Sensors and Actuators;2010-08
3 YU Jicheng1, GUO Naifei1,2, LIU Qiu1 (1 College of Life Science, Dalian Nationalities University, Dalian 116600; 2.College of Food Science, Shenyang Agricultural University, Shenyang 110161);Application of Enzyme Immunoassay in Safety Detection of Fruits and Vegetables[J];Journal of Changjiang Vegetables;2008-14
4 WANG Qing~1,WANG Xi-Bo~1,XU Long-Qiang~1,YU Wei-Ling~1,YU Hong~2,ZHANG Wen-Qing~2~1Department of Clinical Laboratory,Affiliated Hospital,~2Department of Medicl Microbiology,Medical College,Qingdao University,Qingdao 266003,China;Recombinant PP150 of human cytomegalovirus as antigene for colloidal gold immuno-dot assay to detect HCMV-IgM in sera[J];Journal of The Fourth Military Medical University;2005-19
5 LIANG Ai-hui~1,ZOU Ming-jing~2,JIANG Zhi-liang~(1,2),JIN Wen-ying~1(1.Department of Material and Chemical Engineering,Guilin Universityof Technology,Guilin 541004,China;2.Department of Resource and Environmental,Guangxi Normal University,Guilin 541004,China);Immunoassay of Pesticide Environmental Endocrine Disruptors[J];Analysis and Testing Technology and Instruments;2006-02
6 WANG Wei-ping1,WU Yan1,HE Li-jun2,LU Kui2(1.Institute of Engineering Mechanics,Yellow River Institute of Hydraulic Research,Zhengzhou 450003,China;2.School of Chemistry and Chemical Engineering,Henan University of Technology,Zhengzhou 450052,China);Application of Bioenzyme Techniques on Rapid Determination of Pesticide Residues[J];Analysis and Testing Technology and Instruments;2008-02
7 MU Qing-Lin* 1,FANG Jie1,WAN Han-Xing2,WANG Xiao-Hua1,CAO Liu-Yan1,ZHANG Qing-Hong1 1(Zhejiang Provincial Zhoushan Marine Ecological Environmental Monitoring Station,Zhoushan 31600) 2(College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100);Determination of Diarrhetic Shellfish Poisoning in Shellfishes by Liquid Chromatography with Tandem Mass Spectrometry[J];Chinese Journal of Analytical Chemistry;2011-01
8 BAO Hua-jun,WU Ni,SHI Hai-yan,WANG Ming-hua (Department of Pesticide Science,College of Plant Protection,Nanjing Agricultural University,Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects,Ministry of Agriculture,Nanjing210095);An Enzyme-Linked Immunosorbent Assay for Fluazifop-p-butyl[J];Journal of Analytical Science;2010-06
9 Meng Weishuai,Guan Rongfa,Ren Junen,Huang Tianye,Liu Bin(College of Life Sciences,China Jiliang University,Hangzhou 310018,China);Advances Research in Food Allergen Measurement Analysis Technology[J];Guangdong Chemical Industry;2010-04
10 XU Huan,DONG Bin,LIN Yue-xia,TIAN Su-juan,DU Xin-e(School of Life and Biopharmacology,Guangdong Pharmaceutical College,Guangzhou,Guangdong 510006,China);Preparation of colloidal gold nanoparticles and size diameter derermination[J];Journal of Guangdong Pharmaceutical College;2010-02
China Proceedings of conference Full-text Database 2 Hits
1 LI Jing, LIU Yingsheng, LI Long (Department of Health Toxicology and MOE Key Lab of Environment and Health, School of Public Health,, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030 , China);The Apoptotic PC12 Cells Induced by Domoic Acid and the Effect of Domoic Acid on the Cell Cycle and Reactive Oxygen Species in PC12 Cells[A];[C];2008
2 WEI Feng, ZHAO Shou-cheng, LI Da-zhi (Liaoning Entry - Exit Inspection and quarantine Bureau of PRC, Dalian 116001, China; Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China);Liquid Chromatography with Electrospray Ion - trap Mass for the Determination of Domoic Acid in Shellfish[A];[C];2004
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 DU Wei,LU Dou-ding(Second Institute of Oceanography,SOA,Key Laboratory of Marine Ecosystem and Biogeochemistry,SOA,Hangzhou 310012 China);Harmful effects and detection of toxic algae and their algal toxins[J];Journal of Marine Sciences;2008-02
2 DING Jun (Key Laboratory of Maricultural Ecology, Ministry of Agriculture, PRC,Dalian Fisheries Univ , DaLian 116023,China);A review: paralytic shellfish poisoning and diarrhetic shellfish poisoning in red tide poison[J];Journal of Dalian Fisheries University;2001-03
3 XU Ji-Lin1,YANG Jia-Feng2,YAN Xiao-Jun1,WANG Ya-Jun1,YOU Zhong-Jie2,LI Hai-Ying1,TONG Ling11(Key Laboratory of Applied Marine Biotechnology,Ministry of Education,Ningbo University,Ningbo 315211)2(Ningbo Academy of Ocean and Fishery,Ningbo 315100);Determination of Free Cortisol in Marine Fish Sera Samples by Liquid Chromatography-Mass Spectrometry[J];Chinese Journal of Analytical Chemistry;2008-07
4 LI Zhen-hua1,2,SI-KAN-DA-ER Mai-he-mu-ti1,WEI Dong2,3,ZHANG Nai-sheng2(1.College of Animal Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,China;2.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;3.Department of Veterinary Medicine,North Hebei University,Zhangjiakou 075000,China);Establishment of the Method of Detecting the Residues of DFLX by Indirect Competitive Enzyme-linked Immunosorbent Assay[J];China Animal Husbandry & Veterinary Medicine;2007-06
5 Zheng Shuzhen; Lin Xiao(Guangzhou Institute of Chemistry, The Chinese Academy of Sciences, Guangzhou China);Paralytic shellfish poisoning[J];Guangzhou Chemistry;1995-02
6 Yu Ruozhen et al;DEVELOPMENT OF IMMUNOASSAY FOR SMALL MOLECULAR ENVIRONMENTAL POLLUTANTS[J];Environmental Engineering;2005-03
7 SHENG Jian-wu, HE Miao, SONG Bao-dong, SHI Han-chang, QIAN Yi (ESPC State Key Joint Laboratory, Department of Environment Science and Engineering, Tsinghua University, Beijing 100084, China);Synthesis of Complete Antigen of Microcystin-LR and Its Antibody Production[J];Environmental Science;2005-03
8 XI Hai-yan,CAI Qiang,HE Miao,SHI Han-chang (State Key Joint Laboratory of Environmental Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China);Detection of Escherichia coli in Wastewater Based on Enzyme Immunoassay[J];Environmental Science;2005-05
9 SHENG Jian-wu,HE Miao,YU Shao-qing,SHI Han-chang,QIAN Yi(ESPC State Key Joint Laboratory,Department of Environment Science and Engineering,Tsinghua University,Beijing 100084,China);Detection of Microcystin-LR in Waters Using Indirect Competitive ELISA[J];Environmental Science;2006-06
10 Hu Haoyan,Tang Jingliang,Wang Yiming,Huang Bei,Chen Yihua, Mao Hongyue.(Zhoushan Marine Ecological Environment Monitoring Station,Zhoushan Zhejiang 316000);Study on paralytic shellfish poisoning in harmful red tides areas of Zhejiang sea-areas[J];Environmental Pollution & Control;2005-06
China Proceedings of conference Full-text Database 1 Hits
1 Kong Fanzhou, Xu Zijun, Li Qinliang, Zhang Aijun, Zhang Hongliang (North China Sea Monitoring Center, Qingdao 266033);Research on Distribution of Shellfish Toxin and Harmful Red Tide Planktons in North China Sea[A];[C];2004
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved