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The Effects of Physical and Chemical Conditions on Forming Mycelial Pellet of Phanerochaete chrysosporium and Biosorption of Lead

Li Qingbiao\ Wu Juan\ Yang Hongquan\ Deng Xu\ Lu Yinghua (Department of Chemical Engineering,Xiamen University,Xiamen 361005 E mail:kelqb@jingxian.xmu.edu.cn) Hong Liyu\ Cai Lizhe (Research Center of Environment and Science,Xiamen University,Xiamen  
The research on the growth of Phanerochaete chrysosporium in the form of pellet was made in this paper to examine the effects of physical and chemical conditions on the formation of mycelial pellets.The experimental results showed that the microorganism pellets of smooth surface,even size(diameter)and good mechanical properties could be obtained at the follwoing culture conditions:pH4 5,10 6 spores/ml in inoculum,0 1% Tween 80 in medium,high C/N and on the shaker of 150r/min for 3d.Using P.chrysosporium mycelial pellets in the biosorption of Pb 2+ from aqueous solution,the uptake reached the maximum when the diameter of pellet was in the range of 1 5—1 7mm.When the pellets were pretreated by boiling in 0 2mol/L NaOH for 40min,the Pb 2+ uptake was far higher than the pellets without pretreatment,and the removal efficiency could reach above 95%.The research results showed that the removal of Pb 2+ from aqueous solution by P.chrysosporium mycelial pellets was practical.
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