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《Environmental Pollution & Control》 2010-11
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Improved acute toxicity test method for luminescent bacteria

MA Yong1,HUANG Yan2,JIA Yuling3,YU Haiyan1,CAI Qiang3,WANG Ang4,CHEN Mingyuan4.(1.Zhejiang Environmental Monitoring Center,Hangzhou Zhejiang 310012;2.Environmental Science Research and Design Institute of Zhejiang Province,Hangzhou Zhejiang 310007; 3.Yangtze Delta Region Institute of Tsinghua University,Jiaxing Zhejiang 314000;4.Hangzhou Environmental Monitoring Center,Hangzhou Zhejiang 310007)  
Using the traditional acute toxicity test methods for luminescent bacteria, the results are often unstable with poor reproducibility. Based on the optimized experimental conditions and also with two extra parameters, an improved test method was developed. The improved test method, which was less affected by the bacteria type and sample handling, was employed to measure the acute toxicities of three different luminescent bacteria (Vibro fischeri NRRL B—11177, Vibrio qinghaiensis sp. Q67, Photobacterium phosphorem 502); the results were more reproducible, stable and precise relative to those of the traditional methods. The paper also presents some suggestions to the amendment of GB testing standard for luminescent bacteria.
【CateGory Index】: X832
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