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《Acta Scientiae Circumstantiae》 1990-03
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STUDIES ON ACTIVE OXYGEN RADICALS FROM HUMAN PMN RESPIRATORY BURST INDUCED WITH FLUORIDE BY SPIN TRAPPING TECHNIQUE

Wang Yingyan (Beijing Municipal Institute of Environmental Protection) Zhao Baolu, Li Xiaojie, Hou Jingwu, Xin Wenjuan (Institute of Biophysics, Academia Sinica, Beijing)  
By means of ESR spectrum technique, it was demonstrated that F~ stimulated human PMN respiratory burst to generate active oxygen radieals (O2 and ·OH) and to consume O2. The results using spin trapping technique with DMPO as the trapping agent showed that PMN produced DMPO-OOH under high concentration of F- and DMPO-OH under low eoncentration of F-. Under medium concentration of F", DMPO-OOH was first produced and the DMPO-OH was produced later as the concentration of F~ decreased. The results of SOD and catalase test suggested that active oxygen radicals generated from PMN stimulated by F" mainly originated from O2. By spin probe oximetry method of CTPO and CrOX it was indicated that O2 consumed was from intracellular medium.
【Fund】: 国家自然科学基金资助课题
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