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《Acta Scientiae Circumstantiae》 2004-05
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Studies on the effects and corresponding mechanism of Hg~(2+) on the activity of soil acid phosphatase

XU Dongmei, LIU Guangshen, WANG Liming, LIU Weiping (Institute of Environmental Science, Zhejiang University, Hangzhou 310029)  
By fluorescence and differential UV-absorption spectra, the effects of Hg~(2+) on the activity and kinetic property of soil acid phosphatase were studied in soil culture under laboratory conditions, the corresponding mechanism was discussed as well. The obtained results revealed that Hg~(2+) had significant influences on the above characteristics of soil acid phosphatase. Data for kinetic studies suggested that the interaction between Hg~(2+) and soil acid phosphatase probably be mixed types of non-and anti-competitive inhibition interactions, whereas Hg~(2+) was an anti-competitive inhibitor of acid phosphatase from wheat germ. In addition, Hg~(2+) changed the microenvironment of tryptophan and tyrosine groups of soil acid phosphatase, which were active sites of the enzyme and consequently inhibited the activity of the enzyme.
【Fund】: 国家重点研究发展规划 ( 973 )项目 ( 2 0 0 2CB4 1 0 80 0 )
【CateGory Index】: X53
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