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《Acta Scientiae Circumstantiae》 2008-07
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Effect of cadmium on the expression of Fas and caspase-3 mRNA in chicken anterior pituitary

HE Baoxia1,2,FU Yequan2,LI Jinlong1,XU Shiwen1,1.Department of Veterinary Medicine, Northeast Agricultural University, Harbin 1500302.Department of Animal Science, Zhoukou Vocational and Technical College, Zhoukou 466001  
The effect of cadmium chloride on cell apoptosis and the expressions of Fas and caspase-3 genes were explored in a hen model, Ninety normal Hyline white laying hens, aged 50 days, were randomly allocated to 3 groups of 30. The hens were fed 0 mg·kg-1 CdCl2 in the control group, 140 mg·kg-1 CdCl2 in the low-dosage cadmium group and 210 mg·kg-1CdCl2 in the high-dosage cadmium group. The anterior pituitary glands of the hens were removed after 20 d, 40 d and 60 d and the levels of cell apoptosis were detected by TUNEL and the expressions of Fas and caspase-3 genes were measured by RT-PCR. The results indicated that cadmium chloride can lead to apoptosis of pituicytes and overexpression of Fas and caspase-3 genes. At the same time point, cell apoptosis rates and gene expression showed variance (p0.01) in both test groups compared with control and the low-dosage group changed greatly both the expression of Fas, caspase-3 mRNA and cell apoptosis rates. The data indicated that cadmium chloride could induce apoptosis of anterior pituitary cells. The expression of Fas and caspase-3 mRNA have the same trend as apoptosis with the extension of time.
【Fund】: 黑龙江省教育厅资助项目(No10551038)~~
【CateGory Index】: X174
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