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《Journal of Environment and Health》 2002-02
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Studies on Un-fluorescent Staining Methods in Comet Assay

ZHAO Rong, HENG Zheng_chang. Department of Environmental Health, Huaxi College of Public Health, Sichuan University, Chengdu 610041  
Objective To develop an un_fluorescent staining method in Comet Assay. Methods Some special un_fluorescent DNA staining methods, Feulgen, Methyl Green, Stains_all and Silver_Stain were tested in Comet Assay and the comparison studies with EB stain were carried out. Results Among the stain methods tested, just the silver_stain could give a satisfactory effect: legible, ease to judge the stained area of the comets head and tail, permanent, sensitive, could make little segment of DNA be stained, which couldnt be stained by EB stain, the length of comets head and tail, were over 2 times of that of EB stain, safety and economical. Conclusion The silver_stain method discarded the defects of EB stain, so that it could replace EB stain and make comet assay possible to be extended.
【Fund】: 国家自然科学基金资助 (39670625)
【CateGory Index】: R994.6
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【References】
Chinese Journal Full-text Database 4 Hits
1 YUAN Yuzhe LI Xudong WANG Yuezhao (Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041);Research on Usage of Biological Macromolecular in Environment Contamination[J];World Sci-tech R & D;2004-02
2 ZHENG Xiangyu 1, ZHANG Taiping 1, LIU Zhiqiang 2, PAN Weibin( 1Department of Environmental Science and Technology,South China University of Technology,Guangzhou 510640, China; 2Department of Chemical Engineering,Hunan University of Science and Technology,Xiangtan 411201, China).;A review on pollutant's carcinogenicity-mutagenicity-teratogenicity in water with biomonitoring[J];Chinese Journal of Ecology;2004-04
3 Zhao Rong, Heng Zhengchang Department of Environmental Health , Huaxi College of Public Health , Sichuan University , Chengdu 610041,China;Improvement of the silver-stain and it's test and verification in gomet assay[J];Journal of Hygiene Research;2003-02
4 Zhang Qing bi,Gan Zhong lin,Chen zhi qun,et al Department of Prophylactic Medicine, Luzhou Medical College Luzhou 646000,China;Study of Organic Extracts from Water Samples on DNA Damage of Primary Hepatocytes Cells with Comet Assay[J];Journal of Preventive Medicine Information;2003-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Zun-zhen, HENG Zheng-chang, LIAO Yan, ZHAO Rong (Department of Environment Health, West China University of Medical Sciences, Chengdu 610041,China);STUDY ON DNA DAMAGE INDUCED BY INDIRECT MUTAGENS USING COMET ASSAY IN TESTICULAR CELLS IN VITRO[J];Carcinogenesis,Teratogenesis and Mutagenesis;2001-01
2 Liu Xiaoying Zheng Shilian Deng Chuanjun Sun Li (Department of Biology. Anhui University Hefei 230039) Song Degui (Hefei Biochemical Pharmaceutical Factory 230031);Research on the purification of Gyp and its properties[J];JOURNAL OF ANHUI UNIVERSITY(NATURAL SCIENCES);1996-04
3 Pan Fan Shen Shuyu Ding Zaifu Lu Sayin (Department of Biology, Experiment Center, Anhui Uneversity,Hefei,230039 PRC) Chen Ruijuan Pan Kehao (Department of Biology, Anhui University);Study on Production of γ-Linolenic Acid by Mortierella sp.[J];Journal of Anhui University(Natural Sciences);1997-02
4 Zhang Ji Zhao YongFang (School of Life Science, Wuhan university, Wuhan 430072);Studies on the Stability of EPA[J];AMINO ACIDS & BIOTIC RESOURCES;1999-04
5 PENG Jia-bei, YANG Ping (College of Pharmacy, Wuhan University, Wuhan 430072);Isolation and Purification of Plasminogen from Human Plasma[J];Amino Acids & Biotic Resources;2002-04
6 JIA Hai-quan, JIN Hong, XU Zhi-qin, WANG Xian-yuan, NAN Wen-kao, LI Pei-bing (Institute of Hygiene and Environmental Medicine, Academy of Military Medical Science, Tianjin 300050, China);Preventive Effects of Soybean Isoflavones on Hematopoietic System of Mice Suffering Irradiation Damage[J];Amino Acids & Biotic Resources;2005-02
7 Zou Guolin Zhai Yihua Yang Xiong Gui Xingfen(College of Life Sciences, Wuhan University, Wuhan 430072, China);Purification and Characterization of Superoxide Dismutase in Leek Chloroplast[J];AMINO ACIDS & BIOTIC RESOURCES;1998-02
8 MIAO Yue-qiu~1,MEI Qiu-hong~1,GU Gong-ping~2,SHEN Qi~1,LU Chang-mei~1,WU Guo-rong~1(1.College of Life Sciences,Nanjing Normal University,Nanjing 210097;2.Nanjing Institute for Comprehensive Utilization of Wild Plant,Nanjing 210042);Optimization for the enzyme hydrolysis of fenugreek neutral heteropolysaccharide and the effects of its products on reproduction of Bifidobacterium adolescentis[J];Journal of Anhui Agricultural University;2006-02
9 yuan Gang Yang AndaoInstitute of Virology, Chinese Academy of Preventive Medicine, Beijing;IMMUNOGENICITY OF MICELLE FORM AND IMMUNE-STIMULATING COMPLEXES PREPARED FROM HEPATITIS B VIRUS SURFACE ANTIGEN[J];Chinese Journal of Virology;1992-01
10 XUE Jun-Zeng(Department of Biology, Hangzhou Teachers'College Hangzhou 310036);Analysis of the Soluble Proteins and Isozymes of the Chinese Mittenhanded Crabs with Shiver Disease[J];Chinese Journal of Zoology;2002-02
China Proceedings of conference Full-text Database 1 Hits
1 LI Xin, WANG Zhang (School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China);Aqueous enzymatic extraction of corn germ oil and recovering protein by nanofiltration[A];[C];2005
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Zhang Liyun et al, Department of Environmental Health, Tianjin Medical College;THE GENOTOXIC ACTIVITY OF WASTEWATER, SOIL AND VEGETABLES IN WASTEWATER IRRIGATION AREA IN THE MOUSE BONE MARROW MICRONUCLEUS TEST[J];Carcinogenesis,Teratogenesis and Mutagenesis;1990-04
2 ZHONG Yuan\+1, FENG Shao long\+1, SU Qing\+1, XIE Chao yun\+1, LUO Yu\+1, KONG Zhi ming\+1, WAN Hen qing\+2 (1 State Key Laboratory of Pollution Control and Resource Reuse, Department of Environmental Science and Engineering, Nanjing University, Na;STUDY ON MONITORING GENOTOXICANT POLLUTION IN SURFACE WATER OF YANGZHONG CITY USING MICRONUCLEUS TEST AND COMET ASSAY[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;2000-01
3 TANG Ming-de, CHEN Lu,YI Yi-zhen (Department Environmental Hygiene of Hunan Medical University,Changshai\ 410078, China);THE MUTAGENIC STUDY OF DRINKING WATER IN FRONT AND BEHIND CHLORINATION OF DIFFERENT STAGE IN THE WATER FACTORY[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;2000-03
4 JIANG Cheng-mei, ZHUANG Ying, ZHAO Hong, WANG Bang-xia, TIAN Lei (Department of Public Health, Bengbu Medical College, Bengbu 233003, China);STUDIES ON GENOTOXICITY OF NON-VOLATILE ORGANIC CHEMICALS IN HUAI RIVER WATER[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;2000-03
5 ZHANG Zun-zhen, HENG Zheng-chang, LIAO Yan, ZHAO Rong (Department of Environment Health, West China University of Medical Sciences, Chengdu 610041,China);STUDY ON DNA DAMAGE INDUCED BY INDIRECT MUTAGENS USING COMET ASSAY IN TESTICULAR CELLS IN VITRO[J];Carcinogenesis,Teratogenesis and Mutagenesis;2001-01
6 HUANG Mang li, XIA Yi wu, XIONG Wei, WU Yi fan (Pharmacological Division of Hubei Institute for Drug Control, Wuhan 430064, China);STUDY OF MUTAGENICITY AND TERATOGENICITY OF STALK AND LEAF OF TRIPTERYGIUM WILFORDII HOOK. F[J];Carcinogenesis,Teratogenesis and Mutagenesis;2001-03
7 YANG Jian - yi , LI Li , PENG Yun , et al ( Shanxi Medical University, Taiyuan 030001, China);DNA Damage Caused by low Dose Methotrexate and Protective Effect of Folinic acid[J];Carcinogenesis,Teratogenesis and Mutagenesis;2004-01
8 JIN Long_jin,LOU Zhe_feng,DONG Jie_ying,et al (Central Laboratory of Biology,Wenzhou Medical Collage,Wenzhou325027,China);Effects of Cadmium and Mercury on DNA Damage of Mice Bone Marrow Cell and Testicle Germ Cell in vitro[J];Carcinogenesis,Teratogenesis and Mutagenesis;2004-02
9 Yu Ruilan ,Guo YehuaPearl River Fisheries Research Institute, Guangzhou 510380;STUDY ON THE MUTAGENICITY OF NONVOLATILE ORGANICPOLLUTANTS IN CHEMICAL INDUSTKRY SEWAGE[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;1995-03
10 Han Shuqing,Yu Dekui,Lu Yan,Wu Lina Environmental Health Department, Tianjin Medical University, Tianjin 300070;STUDY ON THE MUTAGENICITY OF ORGANIC EXTRACT SUB-STANCES(OESs)FROM SOIL,VEGETABLES AND GROUNDWA-TER IN SEWAGE AND GROUNDWATER IRRIGATED AREAS[J];CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS;1995-06
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 LI An-lin et al(Key Laboratory of Food Science and Safety,Ministry of Education,School of Food Science and Technology,Southern Yangtze University,Wuxi,Jiangsu 214036);Protection of Antioxidants Supplement in Oxidative Stress of Rat Fed with High-fat Diet[J];Journal of Anhui Agricultural Sciences;2006-06
2 Cheng Ying,Pei Zongping,Deng Xia,Liang Fengjiao(College of Environment and Spatial Informatics,China University of Ming &Technology,Xuzhou 221008,China);Application and Problems of Biological Monitoring in Water Environment[J];Environmental Science and Management;2008-02
3 TANG Lin1,2,YANG Ting1,2,ZHANG Ze-yong1,2,WANG Wen-bin1,2,LUO Yu-shuang1,2,ZENG Bo-ping1,2 (1.College of Life Sciences,Hunan University of Arts and Science,Changde,Hunan,415000;2.Key Laboratory of Zoology in Hunan Higher Education,Changde,Hunan,415000);Study on acute toxicity and nuclear anomalies in Erythocytes of Monopterus albus by Laundry Detergents[J];Journal of Hunan University of Arts and Science(Science and Technology);2009-04
4 Yan Xuekun Chen Ying Du Jie Zhang Xueqing(Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing,100850);APPLICATION OF SINGLE CELL GEL ELECTROPHORESIS ASSAY IN RADIATION BIODOSE ESTIMATION AND ENVIRONMENT MONITORING[J];Radiation Protection;2009-01
5 Zhu Liang,Sun Wenpeng. (College of Environmental Science and Engineering,Hohai University,Nanjing Jiangsu 210098);Safety assessment for surface waters using the Comet assay[J];Environmental Pollution & Control;2007-04
6 SUN Zeng-rong,LIU Xue-hui,LU Yan,et al.Department of Environmental Health,School of Public Health,Tianjin Medical University,Tianjin 300070,China;DNA Damage in Mice Testicle Cells Induced by Organic Extracts of Fishpond Water Containing Sewage[J];Journal of Environment and Health;2005-06
7 SHI Yan-ling, ZHENG Guang-hong, WANG Lei, et al. State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai 200092, China;Safety Evaluation of Drinking Water and the Related Products[J];Journal of Environment and Health;2007-05
8 XIA Yong,CHEN Jiang,FU Jian-yun,SONG Yan-hua,ZHENG Yun-yan,GAO Xiao-ping.Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou 310051,Zhejiang,China;Toxicological study on pollution in the Qiantang river system[J];Disease Surveillance;2010-10
9 YUAN Yuzhe LI Xudong WANG Yuezhao (Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041);Research on Usage of Biological Macromolecular in Environment Contamination[J];World Sci-tech R & D;2004-02
10 HUANG Yongjie,LIU Dengyi,WANG Youbao,WANG Xingming,LI Jing (Center of Biodiversity Study,College of Life Science,Anhui Normal University,Wuhu 241000,China);Heavy metals accumulation by hydrophytes[J];Chinese Journal of Ecology;2006-05
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Heng Zhengchang\ Zhang Zunzhen (Dept.of Environmental Health,West china University of Medical sciences,Chengdu 610041);Studies of DNA damages induced by 2,4 dichloro 6 aminophenol in V79 cells[J];JOURNAL OF HEALTH TOXICOLOGY;1997-02
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