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《Journal of Environment and Health》 2004-04
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Effect of Static Magnetic Field on Growth of Rat Embryonic Spinal Cord Neurons in Vitro

DUAN Li-rong, ZHANG Zhi-jian, WU Quan-yi, et al. Department of Hygiene, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212001, China  
Objective To study the effects of static magnetic field (SMF) on development of rat embryonic spinal cord neurons. Methods Primary cultured embryonic spinal cord neurons of Wistar rat were exposed to 1.0, 10.0, 50.0, 100.0 and 200.0 mT static magnetic field. The morphological structure, cell's differentiation and proliferation of the embryonic spinal cord neurons were observed and the contents of MDA, superoxide dismutase (SOD) and protein contents in the neurons were determined. Results Static magnetic field at density of 50-200.0 mT could inhibit the differentiation and proliferation of the cells and the phenomena such as cell aggregation, detouchment of some cells, decrease of clone-formation rate and the size of the cells were observed. The contents of MDA in the cells were increased, while the activities of SOD and the level of protein were decreased. Conclusion Static magnetic field might damage the development of embryonic spinal cord neurons by enhancing the lipid peroxidation.
【Fund】: 江苏省教育厅自然科学基金资助项目(01kJD310006)
【CateGory Index】: R329
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