Effect of Microwave Electromagnetic Fields on Activity of Energy Metabolism Cytochrome Oxidase in Cerebral Cortical Neurons of Postnatal Rats
WANG Qiang, CAO Zhao-jin, BAI Xue-tao. Institute for Environmental Health and Related Product Safety, China Center for Disease Control and Prevention, Beijing 100021, China
Objective To investigate the effect of low intensity microwave electromagnetic fields (EMF) on energy metabolism of cerebral cortical neurons of postnatal rats. Methods The cultured neurons were exposed to low intensity 900 MHz continuous microwave EMF (SAR=0.38-3.22 mW/g) 2 hours a day for 4 to 6 consecutive days. The activity of cytochrome oxidase(CCO) was taken as the index for energy metabolism level. Results The results of cytochemistry showed that compared to the sham-exposed, a significant decrease of cytochrome oxidase activity in exposed neurons(0.38-3.22 mW/g P0.001) had been observed with a trend of dose-effect relationship (P0.05). Conclusion Long term and continuous exposure to 900 MHz microwave EMF at intensity of 0.38-3.22 mW/g may decrease the energy metabolism of cerebral cortical neurons of postnatal rat and has a functional accumulation effect, this observed effect should be classified as non-thermal effect.