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《Journal of Navy Medicine》 2005-03
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Changes of glycosylation in the course of HL - 60 cell differentiation induced by all - trans retinoic acid(AT - RA)

FENG Bing, ZHANG Ji-ren, XIAO Ming-xing, et al (Department of Nephrology, No. 411 Hospital of PLA, Shanghai 200081, China)  
Objective: To investigate changes in the carbohydrate structures of HL-60 cell surface during AT- RA induced differentiation. Methods: Five kinds of Na125I - binding lectins, i. e. ConA, WGA, UEA- 1, PNA, RCA, were used to detect changes in the carbohydrate structure at various stages of differentiation. Results: The levels of ConA, UEA- 1, WGA receptors apparently increased, while the binding of PNA obviously decreased during HL - 60 cell differentiation. Conclusions: The D - Gal - N - GalNAc structures were mainly expressed in the early differentiation stages of HL- 60, and the amount of expression decreased significantly, when cell differentiation came to its mature stage. As a result, D - Glc or D - Man - associated carbohydrate structures on the cell surface could be seen quite obviously
【CateGory Index】: R733.7;
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