Preparation of ~(99m)Tc-HYNIC-Annexin V
LAN Xiao-Li, ZHANG Yong-Xue (Department of Nuclear Medicine,Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022)
99mTc-HYNIC-Annexin V was prepared for apoptosis radionuclide imaging in vivo. The expression plas-mid of human Annexin V was constructed and expressed in E. coli.. HYNIC was prepared and used as bifunctional chelating agent for 99mTc labeling. The radiochemical purity, radiolabeling yield and stability of 99mTc-HYNIC-Annexin V were studied. The coding sequence of human Annexin V gene was successfully cloned into the expression vector, and highly expressed in E. coli. A protein with a molecular weight of about 36kD could be in-duced and confirmed by Western Blotting and SDS-PAGE. Annexin V could be labeled with 99mTc using HYNIC,and showed high radiochemical purity of more than 90%. The radiolabeling yield could reach more than 90%. The reac-tion condition was moderate. It suggested that 99mTc-HYNIC-Annexin V may be a promising agent for apoptosis im-aging and clinical application.
【CateGory Index】： R817;